Detection of A/G Single Nucleotide Alteration in RNA Using Base-discriminating Fluorescent Oligodeoxynucleotides

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  • Akimitsu Okamoto
    Department of Synthetic Chemistry and Biological Chemistry, Faculty of Engineering, Kyoto University and SORST, Japan Science and Technology CorporationKyoto
  • Kazuki Tainaka
    Department of Synthetic Chemistry and Biological Chemistry, Faculty of Engineering, Kyoto University and SORST, Japan Science and Technology CorporationKyoto
  • Isao Saito
    Department of Synthetic Chemistry and Biological Chemistry, Faculty of Engineering, Kyoto University and SORST, Japan Science and Technology CorporationKyoto

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Abstract

<jats:title>Abstract</jats:title> <jats:p>We report the fluorescence properties of oligodeoxynucleotides (ODNs) containing a base-discriminating fluorescent nucleoside, benzopyridopyrimidine (BPP), in hybridizing with RNAs possessing different bases opposite BPP. BPP-containing ODN selectively exhibited a strong fluorescence when the RNA base opposite BPP was A, whereas the fluorescence of BPP-containing ODN hybridized with RNA possessing G opposite BPP was very weak. BPP acts as a good base-discriminating fluorescent nucleoside for A/G single base alteration of RNA.</jats:p>

Journal

  • Chemistry Letters

    Chemistry Letters 32 (8), 684-685, 2003-07-07

    Oxford University Press (OUP)

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