Kinetic Analysis of Enzymatic Hydrolysis of Raw Starch by Glucoamylase Using an Amperometric Glucose Sensor

• Tatsumi Hirosuke

  Department of Bioscience, Fukui Prefectural University

• Katano Hajime

  Department of Bioscience, Fukui Prefectural University

Abstract

An amperometric glucose sensor has been introduced to study the kinetics of enzymatic hydrolysis of raw starch by glucoamylase in a thick raw starch suspension. The initial rate of the hydrolysis increased with increasing amount of enzyme to approach a saturation value, whereas it was proportional to the amount of substrate. The dependence can be explained by the rate equation based on the Langmuir adsorption isotherm.

Journal

• Chemistry Letters

  Chemistry Letters 33 (6), 692-693, 2004

  The Chemical Society of Japan

Keywords

• General Chemistry

Details

• CRID: 1390001204582834688
• NII Article ID: 10013105672
• NII Book ID: AA00603318
• DOI: 10.1246/cl.2004.692
• ISSN: 13480715
• ISSN: 03667022
• Web Site: https://academic.oup.com/chemlett/article-pdf/33/6/692/56256478/cl.2004.692.pdf
• Text Lang: en
• Data Source:
  • JaLC
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed