Immobilization and Hybridization Behavior of DNA on Poly(ethylene glycol)-<i>block</i>-Poly[2-(<i>N</i>,<i>N</i>-dimethylamino)ethyl methacrylate]-modified Gold Surfaces

• Keitaro Yoshimoto

  Graduate School of Pure and Applied Science, University of Tsukuba

• Shinya Matsumoto

  Graduate School of Pure and Applied Science, University of Tsukuba

• Ryosuke Asakawa

  Department of Materials Science, Tokyo University of Science

• Katsumi Uchida

  Department of Materials Science, Tokyo University of Science

• Takehiko Ishii

  Department of Materials Science, Tokyo University of Science

• Yukio Nagasaki

  Graduate School of Pure and Applied Science, University of Tsukuba

Abstract

Single-stranded DNA (ssDNA) immobilization in a highly oriented manner with controlled ssDNA density on a gold surface was accomplished by surface modification with our original poly(ethylene glycol) (PEG)-block-oligoamine copolymer, followed by immobilization of thiol-ended ssDNA (ssDNA-SH) on the PEG tethered chain surface. As the length of the polyamine segment in the block copolymer increased, the immobilization amount of ssDNA-SH on the gold surface increased. The constructed surface improved the signal of the hybridization of ssDNA-SH with its target ssDNA, as found by surface plasmon resonance (SPR) measurements.

Journal

• Chemistry Letters

  Chemistry Letters 36 (12), 1444-1445, 2007-11-10

  Oxford University Press (OUP)

Keywords

• General Chemistry

Details

• CRID: 1360565169064376448
• NII Article ID: 10020011799
• NII Book ID: AA00603318
• DOI: 10.1246/cl.2007.1444
• ISSN: 13480715
• ISSN: 03667022
• Web Site: https://academic.oup.com/chemlett/article-pdf/36/12/1444/55595432/cl.2007.1444.pdf
• Data Source:
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