Identification of Japanese Tea (Camellia sinensis) Cultivars Using SSR Marker

• Kato Fumiko

  National Institute of Vegetable and Tea Science, National Agriculture and Bio-oriented Research Organization

• Taniguchi Fumiya

  National Institute of Vegetable and Tea Science, National Agriculture and Bio-oriented Research Organization

• Monobe Manami

  National Institute of Vegetable and Tea Science, National Agriculture and Bio-oriented Research Organization

• Ema Kaori

  National Institute of Vegetable and Tea Science, National Agriculture and Bio-oriented Research Organization

• Hirono Hisako

  National Institute of Vegetable and Tea Science, National Agriculture and Bio-oriented Research Organization

• Maeda-Yamamoto Mari

  National Institute of Vegetable and Tea Science, National Agriculture and Bio-oriented Research Organization

Bibliographic Information

Other Title

• 単純反復配列（ＳＳＲ）マーカーを利用したチャの品種識別
• タンジュン ハンプク ハイレツ SSR マーカー オ リヨウシタ チャ ノ ヒンシュ シキベツ

Abstract

We have developed SSR (simple sequence repeats) markers for identification of tea cultivars developed and registered in Japan using nucleotide information from Camellia sinensis. Among 44 primer pairs investigated, 6 primers were found to uniquely identify tea cultivars. The selected markers were polymorphic, showing 4 to 13 different fragments per marker, and a minimum of 2 markers was sufficient to distinguish all 41 cultivars investigated. In this study, we also tested the detection of cultivar contamination. As a model for contaminated samples, ‘Kanayamidori’ was mixed in ‘Benifuuki’. PCR products amplified by SSR marker 11D02T were analyzed by capillary electrophoresis by means of DNA sequencer. The peak derived from ‘Kanayamidori’ was detected even in a mixing ratio of 2% (w/w). This shows that the present method is useful for the identification of Japanese tea cultivars and can be applied to powdered tea.

Journal

• Nippon Shokuhin Kagaku Kogaku Kaishi

  Nippon Shokuhin Kagaku Kogaku Kaishi 55 (2), 49-55, 2008

  Japanese Society for Food Science and Technology

Keywords

• tea
• cultivar identification
• SSR marker

Details

• CRID: 1390282681385628544
• NII Article ID: 10021178175
• NII Book ID: AN10467499
• DOI: 10.3136/nskkk.55.49
• COI: 1:CAS:528:DC%2BD1cXjsVOguro%3D
• ISSN: 18816681
• ISSN: 1341027X
• NDL BIB ID: 9372591
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I9372591
• Text Lang: ja
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed