Pulsed Ultrasound Effect on DNA Polymerase Reaction Monitored on a QCM

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  • Takayoshi Kawasaki
    Department of Biomolecular Engineering and Frontier Collaborative Research Center, Tokyo Institute of Technology
  • Momoko Toyoda
    Department of Biomolecular Engineering and Frontier Collaborative Research Center, Tokyo Institute of Technology
  • Yu Hoshino
    Department of Biomolecular Engineering and Frontier Collaborative Research Center, Tokyo Institute of Technology
  • Yoshio Okahata
    Department of Biomolecular Engineering and Frontier Collaborative Research Center, Tokyo Institute of Technology

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Abstract

<jats:title>Abstract</jats:title> <jats:p>DNA polymerase reaction under pulsed ultrasound irradiation was investigated on a DNA-immobilized quartz-crystal microbalance (QCM), and the 240-kHz ultrasound irradiation pulsed at 20 s−1 increased the polymerization reactivity owing to the increase of the stability of the DNA/polymerase/monomer complex, although the continuous irradiation decreases the stability.</jats:p>

Journal

  • Chemistry Letters

    Chemistry Letters 38 (6), 538-539, 2009-04-25

    Oxford University Press (OUP)

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