Development of a HPLC-ESR Spin-trapping System for Post-column On-line Detection of Superoxide Radical Scavenging Ability of Column Eluates

• Yasuhiro Sakurai

  Department of Biomolecular Engineering, Kyoto Institute of Technology

• Kiyoshi Kawai

  Department of Biomolecular Engineering, Kyoto Institute of Technology

• Rushiru Komatsu-Watanabe

  Department of Biomolecular Engineering, Kyoto Institute of Technology

• Chie Morimoto

  Faculty of Pharmacy, Yasuda Women’s University

• Shunji Sakamoto

  Department of Nutritional Science, Yasuda Woman’s University

• Akira Nakajima

  Faculty of Medicine, University of Miyazaki

• Kenji Kanaori

  Department of Biomolecular Engineering, Kyoto Institute of Technology

• Kunihiko Tajima

  Department of Biomolecular Engineering, Kyoto Institute of Technology

Abstract

A new HPLC-ESR spin-trapping system for post-column on-line detection of the superoxide radical scavenging activity of column eluates was developed. HPLC-ESR analysis are performed on a mixture of three phenolic compounds, gallic acid (Gal), 4-hydroxycoumaric acid (Cou), and caffeic acid (Caf), by monitoring ESR signal intensity of the superoxide radical adduct of 5,5-dimethyl-1-pyrroline N-oxide (DMPO/O2).

Journal

• Chemistry Letters

  Chemistry Letters 38 (7), 662-663, 2009-05-30

  Oxford University Press (OUP)

Keywords

• General Chemistry

Details

• CRID: 1360565169064726016
• NII Article ID: 10025123787
• NII Book ID: AA00603318
• DOI: 10.1246/cl.2009.662
• ISSN: 13480715
• ISSN: 03667022
• Web Site: https://academic.oup.com/chemlett/article-pdf/38/7/662/55599722/cl.2009.662.pdf
• Data Source:
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