Elution Behavior of Proteins in Capillary Chromatography Using an Untreated Fused-silica Capillary Tube and a Water-Hydrophilic-Hydrophobic Organic Mixture Carrier Solvent

• Masuhara Yuji

  Department of Chemical Engineering and Materials Science, Doshisha University

• Jinno Naoya

  Department of Chemical Engineering and Materials Science, Doshisha University

• Hashimoto Masahiko

  Department of Chemical Engineering and Materials Science, Doshisha University

• Tsukagoshi Kazuhiko

  Department of Chemical Engineering and Materials Science, Doshisha University

Abstract

We examined the elution behavior of isoluminol isothiocyanate (ILITC)-labeled proteins (alcohol dehydrogenase (ADH) and bovine serum albumin (BSA)) in a capillary chromatography system using an untreated fused-silica capillary tube and a water–acetonitrile–ethyl acetate mixture carrier solution. The mixture of ILITC and ILITC-labeled protein (ADH or BSA) was analyzed using this system with a chemiluminescence detector; the ILITC and the labeled protein were separated in this order with an organic solvent-rich carrier solution, while they were eluted in the reverse order with a water-rich carrier solution. In addition, the mixture of ILITC, ILITC-labeled ADH, and ILITC-labeled BSA was added to this system, and the analytes of the mixture were separated and confirmed with the individual peaks on the chromatograms.

Journal

• Chemistry Letters

  Chemistry Letters 39 (7), 688-689, 2010

  The Chemical Society of Japan

Keywords

• General Chemistry

Details

• CRID: 1390001204586595712
• NII Article ID: 10027182282
• NII Book ID: AA00603318
• DOI: 10.1246/cl.2010.688
• COI: 1:CAS:528:DC%2BC3cXpsFensrk%3D
• ISSN: 13480715
• ISSN: 03667022
• Web Site: https://academic.oup.com/chemlett/article-pdf/39/7/688/55603388/cl.2010.688.pdf
• Text Lang: en
• Data Source:
  • JaLC
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed