Rational Design of a Fluorescence-amplified Aptasensor Based on Enzyme-assisted Target Recycling Strategy

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Author(s)

    • NAKAO Shingo
    • Division of Materials Science, Graduate School of Pure and Applied Sciences, University of Tsukuha
    • OISHI Motoi
    • Division of Materials Science, Graduate School of Pure and Applied Sciences, University of Tsukuha

Abstract

A fluorescence-amplified sensing system composed of a fluorophore-labeled adenosine DNA-aptamer/quencher-labeled capture DNA duplex, assistant DNA, and exonuclease III (Exo III) was designed and prepared. The newly constructed enzyme-assisted aptasensor exhibited amplification of fluorescent signal in response to adenosine (target) through an Exo III-assisted target recycling process.

Journal

  • Chemistry Letters

    Chemistry Letters 42(10), 1122-1124, 2013-10-05

    The Chemical Society of Japan

References:  28

Codes

  • NII Article ID (NAID)
    10031201282
  • NII NACSIS-CAT ID (NCID)
    AA00603318
  • Text Lang
    ENG
  • Article Type
    SHO
  • ISSN
    03667022
  • Data Source
    CJP  J-STAGE 
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