Species Identification and Functional Components of Wild Mushrooms from Rakuno Gakuen University, Hokkaido

• Sugawara Ryota

  Department of Food Science and Human Wellness, Rakuno Gakuen University

• Yamada Sayumi

  Department of Food Science and Human Wellness, Rakuno Gakuen University

• Tu Zhihao

  Department of Food Science and Human Wellness, Rakuno Gakuen University

• Sugawara Akiko

  Department of Food Science and Human Wellness, Rakuno Gakuen University

• Hoshiba Toshihiro

  Department of Food Science and Human Wellness, Rakuno Gakuen University

• Yamauchi Masahito

  Department of Civil and Environmental Engineering, National Institute of Technology, Kagoshima College

• Yamaguchi Akihiro

  Department of Food Science and Human Wellness, Rakuno Gakuen University

Bibliographic Information

Other Title

• 道央圏に自生するキノコの同定と機能性成分の含有量
• ドウオウケン ニ ジセイ スル キノコ ノ ドウテイ ト キノウセイ セイブン ノ ガンユウリョウ

Abstract

This study focuses on the identification and characterization of wild mushrooms grown on the campus of Rakuno Gakuen University, using DNA sequencing and analysis of functional metabolites. Forty-eight samples of fruiting bodies were collected between June 2012 and November 2014. The samples were hot-air dried and powdered, and subsequently extracted with alkaline hot water to obtain beta-glucans, or 50% ethanol for total polyphenols (TPP) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity. Functional metabolites were assessed by colorimetric assays, using Congo Red reagent for beta-glucans, Folin-Denis reagent for TPP level, and DPPH radical scavenging for antioxidant activity. Total DNA was extracted from each sample and subjected to DNA sequencing for the inter spacer regions of ribosomal RNA gene. Sequencing analysis identified 27 independent species or genus of mushrooms. Beta-glucan contents showed clear differences between species. The TPP levels and DPPH radical scavenging activity showed similar tendencies and were significantly correlated (p<0.0001).

Journal

• Nippon Shokuhin Kagaku Kogaku Kaishi

  Nippon Shokuhin Kagaku Kogaku Kaishi 62 (9), 445-453, 2015

  Japanese Society for Food Science and Technology

Keywords

• wild mushroom
• species identification
• genetic analysis
• beta-glucan
• food function

Details

• CRID: 1390282681384036608
• NII Article ID: 130005105544
• NII Book ID: AN10467499
• DOI: 10.3136/nskkk.62.445
• ISSN: 18816681
• ISSN: 1341027X
• HANDLE: 10659/4940
• NDL BIB ID: 026741808
• Web Site: https://agriknowledge.affrc.go.jp/RN/2010900231
• Web Site: https://rakuno.repo.nii.ac.jp/records/1419
• Web Site: http://id.ndl.go.jp/bib/026741808
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I026741808
• Web Site: https://www.jstage.jst.go.jp/article/nskkk/62/9/62_445/_pdf
• Text Lang: ja
• Data Source:
  • JaLC
  • IRDB
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed