Plasmalogen mediates integration of adherens junction

• Takanori Takahashi

• Masanori Honsho

• Yuichi Abe

• Yukio Fujiki

Abstract

Ether glycerolipids, plasmalogens are found in various mammalian cells and tissues. However, physiological role of plasmalogens in epithelial cells remains unknown. We herein show that synthesis of ethanolamine-containing plasmalogens, plasmenylethanolamine (PlsEtn), is deficient in MCF7 cells, an epithelial cell line, with severely reduced expression of alkyl-dihydroxyacetonephosphate synthase (ADAPS), the second enzyme in the PlsEtn biosynthesis. Moreover, expression of ADAPS or supplementation of PlsEtn containing C18-alkenyl residue delays the migration of MCF7 cells as compared to that mock-treated MCF7 and C16-alkenyl-PlsEtn-supplemented MCF7 cells. Localization of E-cadherin to cell–cell junctions is highly augmented in cells containing C18-alkenyl-PlsEtn. Together, these results suggest that PlsEtn containing C18-alkenyl residue plays a distinct role in the integrity of E-cadherin-mediated adherens junction.

Journal

• The journal of biochemistry

  The journal of biochemistry 166 (5), 423-432, 2019-11

  Tokyo : Japanese Biochemical Society

Keywords

• adherens junction
• cell migration
• epithelial cells
• peroxisome
• plasmalogen

Details

• CRID: 1523669554832755584
• NII Article ID: 40022069621
• NII Book ID: AA00694073
• ISSN: 0021924X
• ISSN: 17562651
• DOI: 10.1093/jb/mvz049
• NDL BIB ID: 030083616
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I030083616
• Web Site: http://academic.oup.com/jb/advance-article-pdf/doi/10.1093/jb/mvz049/28937567/mvz049.pdf
• Web Site: http://academic.oup.com/jb/article-pdf/166/5/423/30259866/mvz049.pdf
• Text Lang: en
• NDL Source Classification:
  • ZR2(科学技術--生物学--生化学)
• Data Source:
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN