Thioredoxin pathway in Anabaena sp. PCC 7120 : activity of NADPH-thioredoxin reductase C
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Abstract
<jats:title>Abstract</jats:title> <jats:p>To understand the physiological role of NADPH-thioredoxin reductase C (NTRC) in cyanobacteria, we investigated an NTRC-deficient mutant strain of Anabaena sp., PCC 7120, cultivated under different regimes of nitrogen supplementation and light exposure. The deletion of ntrC did not induce a change in the cell structure and metabolic pathways. However, time-dependent changes in the abundance of specific proteins and metabolites were observed. A decrease in chlorophyll a was correlated with a decrease in chlorophyll a biosynthesis enzymes and photosystem I subunits. The deletion of ntrC led to a deregulation of nitrogen metabolism, including the NtcA accumulation and heterocyst-specific proteins while nitrate ions were available in the culture medium. Interestingly, this deletion resulted in a redox imbalance, indicated by higher peroxide levels, higher catalase activity and the induction of chaperones such as MsrA. Surprisingly, the antioxidant protein 2-CysPrx was downregulated. The deficiency in ntrC also resulted in the accumulation of metabolites such as 6-phosphogluconate, ADP and ATP. Higher levels of NADP+ and NADPH partly correlated with higher G6PDH activity. Rather than impacting protein expression levels, NTRC appears to be involved in the direct regulation of enzymes, especially during the dark-to-light transition period.</jats:p>
Journal
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- The journal of biochemistry
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The journal of biochemistry 169 (6), 709-719, 2021-06
Tokyo : Japanese Biochemical Society
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Details 詳細情報について
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- CRID
- 1522262181130280704
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- NII Article ID
- 40022687787
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- NII Book ID
- AA00694073
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- ISSN
- 0021924X
- 17562651
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- NDL BIB ID
- 031690553
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- Web Site
- http://id.ndl.go.jp/bib/031690553
- https://ndlsearch.ndl.go.jp/books/R000000004-I031690553
- http://academic.oup.com/jb/advance-article-pdf/doi/10.1093/jb/mvab014/36396993/mvab014.pdf
- http://academic.oup.com/jb/article-pdf/169/6/709/40325657/mvab014.pdf
- https://search.jamas.or.jp/link/ui/2022197476
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- Text Lang
- en
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- NDL Source Classification
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- ZR2(科学技術--生物学--生化学)
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- Data Source
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- NDL
- Crossref
- CiNii Articles
- KAKEN