Studies on the cryopreservation of mouse embryos by vitrification マウス胚のガラス化による低温保存に関する研究
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著者
書誌事項
- タイトル
-
Studies on the cryopreservation of mouse embryos by vitrification
- タイトル別名
-
マウス胚のガラス化による低温保存に関する研究
- 著者名
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Conrado A. Valdez
- 著者別名
-
コンラド アントニオ バルデス
- 学位授与大学
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北海道大学
- 取得学位
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獣医学博士
- 学位授与番号
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甲第3079号
- 学位授与年月日
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1992-03-25
注記・抄録
博士論文
目次
- CONTENTS / (0003.jp2)
- GENERAL INTRODUCTION / p1 (0006.jp2)
- PART 1 STUDIES ON SOME FACTORS AFFECTING THE SURVIVAL OF MOUSE EMBRYOS CRYOPRESERVED BY VITRIFICATION / p6 (0011.jp2)
- CHAPTER 1 EFFECTS OF EQUILIBRATION TIME,PRECOOLING AND DEVELOPMENTAL STAGE ON THE SURVIVAL OF MOUSE EMBRYOS CRYOPRESERVED BY VITRIFICATION / p7 (0012.jp2)
- Introduction / p7 (0012.jp2)
- Materials and Methods / p8 (0013.jp2)
- Results / p13 (0018.jp2)
- Discussion / p16 (0021.jp2)
- Summary / p20 (0025.jp2)
- CHAPTER 11 IN VITRO AND IN VIVO DEVELOPMENT OF VITRIFIED-WARMED MOUSE MORULAE / p21 (0026.jp2)
- Introduction / p21 (0026.jp2)
- Materials and Methods / p22 (0027.jp2)
- 1.In vitro experiment / p23 (0028.jp2)
- 2.In vivo experiment / p24 (0029.jp2)
- Results / p25 (0030.jp2)
- Discussion / p28 (0033.jp2)
- Summary / p31 (0036.jp2)
- CHAPTER 111 CONFIRMATION BY CRYO-ELECTRON MICROSCOPY OF THE ABSENCE OF CRYSTALLIZATION USING A VITRIFICATION SOLUTION / p32 (0037.jp2)
- Introduction / p32 (0037.jp2)
- Materials and Methods / p33 (0038.jp2)
- 1.Light microscopic examination / p34 (0039.jp2)
- 2.Cryo-SEM & Freeze fracture EM examination / p35 (0040.jp2)
- Results / p35 (0040.jp2)
- Discussion / p40 (0045.jp2)
- Summary / p43 (0048.jp2)
- PART 11 STUDIES ON THE DEVELOPMENT OF A NEW VITRIFICATION SOLUTION / p44 (0049.jp2)
- CHAPTER 1V EFFECTS OF VARIOUS CRYOPROTECTANTS ON THE SURVIVAL OF NONFROZEN MOUSE BLASTOCYSTS / p45 (0050.jp2)
- Introduction / p45 (0050.jp2)
- Materials and Methods / p46 (0051.jp2)
- 1.Effect of cryoprotectant concentration / p46 (0051.jp2)
- 2.Effect of length of exposure / p47 (0052.jp2)
- 3.Effect of cryoprotectant dilution / p48 (0053.jp2)
- Results / p49 (0054.jp2)
- Discussion / p55 (0060.jp2)
- Summary / p60 (0065.jp2)
- CHAPTER V SUCCESSFUL CRYOPRESERVATION OF MOUSE BLASTOCYSTS USING A NEW VITRIFICATION SOLUTION / p61 (0066.jp2)
- Introduction / p61 (0066.jp2)
- Materials and Methods / p62 (0067.jp2)
- 1.In vitro experiment / p64 (0069.jp2)
- 2.In vivo experiment / p65 (0070.jp2)
- Results / p67 (0072.jp2)
- Discussion / p71 (0076.jp2)
- Summary / p79 (0084.jp2)
- CHAPTER V1 ULTRASTRUCTURAL OBSERVATIONS OF MOUSE BLASTOCYSTS AFTER EXPOSURE IN VITRIFICATION SOLUTION / p80 (0085.jp2)
- Introduction / p80 (0085.jp2)
- Materials and Methods / p80 (0085.jp2)
- 1.Light microscopic examination / p81 (0086.jp2)
- 2.Freeze fracture EM examination / p82 (0087.jp2)
- Results / p82 (0087.jp2)
- Discussion / p87 (0092.jp2)
- Summary / p90 (0095.jp2)
- GENERAL CONCLUSIONS / p102 (0107.jp2)
- SUMMARY / p105 (0110.jp2)
- SUMMARY IN JAPANESE / p108 (0113.jp2)
- ACKNOWLEDGEMENTS / p111 (0116.jp2)
- REFERENCES / p112 (0117.jp2)