Minimally Modified LDL Is an Oxidized LDL Enriched with Oxidized Phosphatidylcholines
-
- Itabe Hiroyuki
- Department of Molecular Pathology, Faculty of Pharmaceutical Sciences, Teikyo University
-
- Mori Masahiro
- Department of Molecular Pathology, Faculty of Pharmaceutical Sciences, Teikyo University
-
- Fujimoto Yasuyuki
- Department of Molecular Pathology, Faculty of Pharmaceutical Sciences, Teikyo University
-
- Higashi Yusuke
- Department of Molecular Pathology, Faculty of Pharmaceutical Sciences, Teikyo University
-
- Takano Tatsuya
- Department of Molecular Pathology, Faculty of Pharmaceutical Sciences, Teikyo University
Search this article
Abstract
The oxidative modification of low-density lipoprotein (LDL) is involved in atherogenesis. Among a variety of modified LDLs mentioned in the literature, so-called minimally modified LDL (MM-LDL) was reported to have pro-atherogenic properties despite minimal changes in its oxidative measures. After treatment of LDL with 1 μM FeSO4 at 4°C for 96h, the resulting MM-LDL showed a slight increase in thiobarbituric acid-reactive substances (TBARS) and little association with macrophages. On the other hand, heavily oxidized LDL, which was prepared by copper-induced oxidation of LDL at 37°C, showed a sharp increase in TBARS and strong association with macrophages. By introducing a fluorometric procedure to detect aldehyde-containing phosphatidylcholines (aldehyde-PCs), we examined the amounts of aldehyde-PCs in modified LDL preparations. Aldehyde-PCs increased to 23.4 pmol/μg protein in MM-LDL, which was more than four-fold higher than in the heavily oxidized LDL. We conclude that MM-LDL is a unique type of oxidized LDL enriched with aldehyde-PCs.
Journal
-
- The Journal of Biochemistry
-
The Journal of Biochemistry 134 (3), 459-465, 2003
The Japanese Biochemical Society
- Tweet
Details 詳細情報について
-
- CRID
- 1390001204964776832
-
- NII Article ID
- 130003418119
- 50000316565
-
- NII Book ID
- AA00694073
-
- COI
- 1:CAS:528:DC%2BD3sXpt1Kitb8%3D
-
- ISSN
- 17562651
- 0021924X
-
- NDL BIB ID
- 6701637
-
- PubMed
- 14561732
-
- Text Lang
- en
-
- Data Source
-
- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
-
- Abstract License Flag
- Disallowed