Electron microscopy in molecular biology : a practical approach

書誌事項

Electron microscopy in molecular biology : a practical approach

edited by J. Sommerville and U. Scheer

(The practical approach series)

IRL, c1987

  • : pbk

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注記

Includes index

内容説明・目次

内容説明

Vast experience has gone into providing this comprehensive guide to analysis at the ultrastructural level of nucleic acids, proteins and macromolecular complexes. Research workers will find it a unique handbook, containing procedures for applications with a wide range of biologically important macromolecules.

目次

  • Abbreviations
  • Preparation of nucleic acids for electron microscopy
  • Denaturation and hybridization of nucleic acids
  • Binding of protein to nucleic acids
  • Chromatin spreading
  • Visualizing active genes
  • Negative staining of protein molecules and filaments
  • Rotary metal shadowing for visualizing rod-shaped proteins
  • Em immunolabelling
  • Autoradiography of spread molecular complexes
  • Hybridization to chromatin and whole chromosome mounts
  • Appendix

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