Monoclonal antibody technology : the production and characterization of rodent and human hybridomas

書誌事項

Monoclonal antibody technology : the production and characterization of rodent and human hybridomas

Ailsa M. Campbell

(Laboratory techniques in biochemistry and molecular biology, v. 13)

Elsevier, 1984

  • : pbk

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注記

Bibliography: p. 243-254

Includes index

内容説明・目次

内容説明

This volume contains detailed, comprehensive advice on rat, mouse and human hybridoma production. It begins with a general introduction, then describes the practical applications of the technology with photographs and protocols for everything from animal dissection to epitope analysis of antigens.

目次

General properties and applications of monoclonal antibodies. Assay techniques. Selection of animals and cell lines. Immunisation. Cell culture requirements for hybridomas. Fusion procedures. Transformation. Selection and cloning. Antibody production and purification. Characterisation of monoclonal antibodies.

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