The practice of quantitative gel electrophoresis
著者
書誌事項
The practice of quantitative gel electrophoresis
（Advanced methods in the biological sciences）
VCH, 1985
- : gw
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注記
Bibliography: p. [197]-209
Includes index
内容説明・目次
内容説明
A practical guide to proven procedures and special methods (including polyacrylamide and agarose electrophoresis, isotachophoresis, and electrofocusing) that are generally applicable to identifying and isolating charged molecules. The book also describes in detail all the equipment required and provides construction plans for those items that are not commercially available. The author has kept theory to a minimum in the interests of readability and usability. The electrophoretic separations he describes are those of native macromolecules as opposed to dissociated and denatured gene products. All the methods have been proved reliable and are used in daily laboratory work. A comprehensive index makes this book an invaluable reference tool for molecular biologists, clinical chemists, pharmaceutical and food chemists, biochemists, and physiologists.
目次
The Concept of an Objectively Defined Optimal Fractionation Route / Selection of a Solvent for Gel Electrophoresis / Concepts and Tools in the Selection of a Separation pH / The Apparatus of Quantitative PAGE / Polyacrylamide / Agarose / Optimization of Stacking pH / Optimization of Trailing and Leading Ion Mobilities in the Stacking Gel / Selection of ''Happiness Conditions'' by Means of Stacking Gels / Physical Characterization of the Species of Interest and its Contaminants by Ferguson Plot / Pore Size Optimization / Electrofocusing on Gels / Preparative Protein Extraction from Gel Slices / Preparative Elution-PAGE and MBE/SDS-PAGE / Multicomponent Analysis / References.
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