The Use of HPLC in receptor biochemistry
著者
書誌事項
The Use of HPLC in receptor biochemistry
（Receptor biochemistry and methodology, v. 14）
A.R. Liss, c1989
- hard
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注記
Includes bibliographies and index
内容説明・目次
内容説明
This text offers a concise background in HPLC methodology and includes illustrative examples of some of the key advances made in the receptor field through application of this chromatographic technique. A brief historical overview puts into perspective the specific state-of-the-art of HPLC laboratory applications that are contained in the book's chapters. Technical contributions discuss how best to select the HPLC mode for a particular biochemical problem, including the most effective use of a particularly sensitive technique, microbore HPLC for synthesis, purification and characterization of synthetic peptides. The text will benefit biochemists and molecular biologists.
目次
- The isolation of endogenous peptides, with an emphasis on their biologic activity
- the application of HPLC to receptor mapping (illustrated with case studies of the structural features of receptors discovered with this technique)
- the use of immunoaffinity chromatography to recover biologically active proteins
- the application of a wide range of HPLC modes to the study of steroid hormone receptors
- a highly detailed strategy for isolating small amounts of proteins and peptides for microsequence analysis.
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