Analytical methods for major and modified nucleosides, HPLC, GC, MS, NMR, UV and FT-IR
著者
書誌事項
Analytical methods for major and modified nucleosides, HPLC, GC, MS, NMR, UV and FT-IR
（Journal of chromatography library, v. 45 . Chromatography and modification of nucleosides ; pt. A）
Elsevier , Distributors for the U.S. and Canada Elsevier Science Pub. Co., 1990
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注記
Includes bibliographical references and index
内容説明・目次
内容説明
Chromatography and Modification of Nucleosides is a four-volume work which provides state-of-the-art chromatography and analytical methods for use in a wide spectrum of nucleic acid modification research. The focus of Part A, is the presentation of advanced methods for modification research on tRNAs, mRNAs, mtRNAs, rRNAs and DNAs. HPLC-UV, GC-MS, NMR, FT-IR and affinity chromatography approaches to nucleic acid modification studies are presented, as are nucleoside, oligonucleotide and nucleic acid isolation techniques. Part B has as its central theme the modified nucleosides of tRNA and the current analytical means for studying tRNA modifications. Modified nucleoside synthesis, function, structural conformation, biological regulation, and occurrence of modification in a wide range of tRNAs are presented, as is a chapter on DNA modification and a chapter on solid phase immunoassay for determining a particular modification. Part C provides the reader with a comprehensive treatment of modified nucleosides as biochemical signals of neoplasia and normal metabolism.
The final volume, Part D, will present structural characterization of unknown nucleosides as well as extensive biochemical, chemical and physical properties of RNA and DNA nucleosides, as a "database" for researchers in the field. Chromatographic methodology will be described for analysis of total modification of tRNAs and DNAs. The chapters are written by leading scientists in their respective fields and present an up-to-date review on the roles of modified nucleosides in nucleic acids which will be extremely useful for workers in chromatography, molecular biology, genetics, biochemistry, biotechnology and the pharmaceutical industry.
目次
Contents of Part A. Introduction and Overview (C.W. Gehrke, K.C. Kuo). 1. Ribonucleoside analysis by reversed-phase high performance liquid chromatography (C.W. Gehrke, K.C. Kuo). 2. HPLC of transfer RNAs using ionic-hydrophobic mixed-mode and hydrophobic-interaction chromatography (R. Bischoff, L.W. McLaughlin). 3. Nucleic acid chromatographic isolation and sequence methods (G. Keith). 4. Affinity chromatography of mammalian tRNAs on immobilized elongation factor Tu from Thermus thermophilus (M. Sprinzl, K.-H. Derwenskus). 5. Structural elucidation of nucleosides in nucleic acids (C.W. Gehrke et al.). 6. Three dimensional dynamic structure of tRNAs by nuclear magnetic resonance spectroscopy (P.F. Agris, H. Sierzputowska-Gracz). 7. Codon recognition: evaluation of the effects of modified bases in the anticodon loop of tRNA using the temperature-jump relaxation method (H. Grosjean, C. Houssier). 8. High-performance liquid chromatography of Cap structures and nucleoside composition in mRNAs (K.C. Kuo et al.). 9. Immunoassays for modified nucleosides of ribonucleic acids (B.S. Vold). 10. Chromatography of synthetic and natural oligonucleotides (H. Eckstein, H. Schott). Subject Index.
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