Gel electrophoresis of nucleic acids : a practical approach
著者
書誌事項
Gel electrophoresis of nucleic acids : a practical approach
(The practical approach series)
IRL Press, c1990
2nd ed
- : pbk
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注記
Includes bibliographical references and index
内容説明・目次
- 巻冊次
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ISBN 9780199630820
内容説明
Now in a new second edition, this popular book includes a wealth of practical information on the much-used techniques of gel electrophoresis. Authoritative and completely current, the volume provides readers with an up-to-date, "how-to" guide to this growing field.
- 巻冊次
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: pbk ISBN 9780199630837
内容説明
The first edition of this title, published in 1982, sold over 13,000 copies worldwide. The second edition takes account of the progress made since then in refining standard methods and applying new ones. One of the major new techniques now covered is pulsed field electrophoresis of DNA. Another key area described in detail is the use of electrophoretic techniques to study the specific interaction of proteins with DNA sequences by foot-printing and gel retardation analysis. These techniques have proved invaluable in pinpointing the exact nature of the interaction of proteins with regulatory DNA sequences. In addition the book now also describes the use of electrophoresis for the purification of synthetic oligonucleotides which are proving to be an essential tool in the areas of molecular biology in general and genetic engineering in particular. In addition the interior of the book has been redesigned to allow greater accessibility to the protocols.
目次
- Gel electrophoresis of RNA, D.Grierson
- gel electrophoresis of DNA, P.G.Sealey
- pulsed field gel electrophoresis, R.Anand
- the electrophoresis of synthetic oligonucleotides, J.W.Efcavitch
- two-dimensional gel electrophoresis of nucleic acids, R de Wachter et al
- gel retardation of nucletic acid-protein interactions, M.M.Garner
- the analysis of sequence-specific DNA-binding proteins in cell extracts, G.H.Goodwin
- electrophoresis of nucleosomes, R.H.Nicholas
- gel electrophoresis of ribonuceotides, A.E.Dahlberg and P.J.Grabowski.
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