Molecular techniques in taxonomy
著者
書誌事項
Molecular techniques in taxonomy
（NATO ASI series, ser. H . Cell biology ; v. 57）
Springer-Verlag, c1991
- : Berlin
- : New York
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注記
"Proceedings of the NATO Advanced Study Institute on Molecular Techniques in Taxonomy held in Norwich (England), July 9-20, 1990"--T.p. verso
内容説明・目次
内容説明
Taxonomy is fundamental to understanding the variety of life forms, and exciting expansions in molecular biology are re- volutionising the obtained data. This volume reviews the ma- jor molecular biological techniques that are applied in ta- xonomy. The chapters are arranged in three main sections:1) Overviews of important topics in molecular taxonomy; 2) Case studies of the successful application of molecular methods to taxonomic and evolutionary questions; 3) Protocols for a range of generally applicable methods. The described techni- ques include DNA-DNA hybridization, DNA fingerprinting, RFLP analysis, and PCR sequencing.
目次
A Prologue.- Past and Future of Taxonomy.- Overviews.- Taxonomy: an Essential Key to Evolutionary Biology.- Variation at the DNA Level: Something for Everyone.- Molecular Systematics at the Species Boundary: Exploiting Conserved and Variable Regions of the Mitochondrial Genome of Animals via Direct Sequencing from Amplified DNA.- Ribosomal RNA Phylogenies.- Evaluating Gene Versus Genome Evolution.- DNA and Higher Plant Systematics: Some Examples From the Legumes.- DNA-DNA Hybridization: Principles and Results.- Satellite DNA.- DNA Fingerprinting.- The Statistical Interpretation of Hypervariable DNAs.- Case Studies.- A Multidimensional Approach to the Evolution and Systematics of Dolichopoda Cave Crickets.- Sperm and Evolution in Drosophila: Morphological and Molecular Aspects.- Episodic Evolutionary Change in Local Populations.- Searching for Speciation Genes in the Species Pair Drosophila Mojavensis and D. Arizonae.- Colonizing Species of Drosophila.- Molecular Taxonomy in the Control of West African Onchocerciasis.- Protocols.- DNA Protocols for Plants.- Prepartion and Visualization of Mitochondrial DNA for RFLP Analysis.- Hybridization of DNA Probes to Filterbound DNA Using the "DIG-DNA Labeling and Detection System Nonradioactive" (Boehringer Mannheim).- DNA Fingerprinting.- The Polymerase Chain Reaction: DNA Extraction and Amplification.- Protocols for 4-Cutter Blots and PCR Sequencing.- Ribosomal RNA Sequencing.- Quantitative DNA:DNA Hybridization and Hydroxyapatite Elution.- A Protocol for the TEACL Method of DNA-DNA Hybridization.- List of Participants.
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