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pt. A ISBN 9780121821074
内容説明
This volume represents the first collection of methodological approaches to P450 investigation which includes the use of molecular biological techniques. It is designed for use by those who study the P450 super family. Methods are presented for the identification and characterization of unique forms of P450 mRNA and protein, for expression of P450s in heterologous systems, for the study of human P450s, and for the characterization of P450s in different tissues.
目次
Site-Directed Mutagenesis, Protein Modification, and Structural Analysis:
D.W. Nebert and D.R. Nelson, P450 Gene Nomenclature Based on Evolution.
T.L. Poulos, Modeling of Mammalian P450s on Basis of P450cam X-Ray Structure.
S.G. Sligar, D. Filipovic, and P.S. Stayton, Mutagenesis of Cytochromes P450cam and b5.
F. Millett and L.M. Geren, Chemical Modification of Interaction between Adrenodoxin and Cytochrome P450scc.
L.S. Kaminsky, D. Dunbar, and W. Lawson, Azidowarfarin as Photoaffinity Probe of Cytochromes P450.
E. Szczesna-Skorupa and B. Kemper, Cell-Free Analysis of Targeting of Cytochrome P450 to Microsomal Membranes.
T. Omura and A. Oto, Biosynthesis and Intracellular Sorting of Mitochondrial Forms of Cytochrome P450.
Heterologous~Expression:
F.J. Gonzalez, T. Aoyama, and H.V. Gelboin, Expression of Mammalian Cytochrome P450 Using Vaccinia Virus.
F.J. Gonzalez, S. Kimura, S. Tamura, and H.V. Gelboin, Expression of Mammalian Cytochrome P450 Using Baculovirus.
B.J. Clark and M.R. Waterman, Heterologous Expression of Mammalian P450 cDNA in COS Cells.
T.D. Porter and J.R. Larson, Expression of Mammalian P450s in Escherichia coli.
J. Doehmer and F. Oesch, V79 Chinese Hamster Cells Genetically Engineered for Stable Expression of Cytochromes P450.
C.L. Crespi, Expression of Cytochrome P450 cDNAs in Human B Lymphoblastoid Cells: Applications to Toxicology and Metabolite Analysis.
F.P. Guengerich, W.R. Brian, M.-A. Sari, and J.T. Ross, Expression of Mammalian~Cytochrome P450 Enzymes Using Yeast-Based Vectors.
Characterization of Genetic Polymorphism and Errors of Metabolism:
N.K. Spurr and C.R. Wolf, Genetic Analysis of Cytochrome P450 System.
Y. Higashi and Y. Fujii-Kuriyama, Functional Analysis of Mutant P450(C21) Genes in a COS Cell Expression System.
M.H. Heim and U.A. Meyer, Genetic Polymorphism of Debrisoquine Oxidation: Restriction Fragment Analysis and Allele-Specific Amplification of Mutant Alleles of CYP2D6.
R. Kato, T. Yasumori, and Y. Yamazoe, Characterization of Human P450IIC Isozyme Using Yeast Expression System.
Immunochemical Methods:
T. Friedberg, W. Kissel, M. Arand, and F. Oesch, Production of Site-Specific P450 Antibodies Using Recombinant Fusion Proteins as Antigens.
U.M. Zanger, ~Immunoisolation of Human Microsomal Cytochromes P450's Using Autoantibodies.
M.P. Manns and E.F. Johnson, Identification of Human Cytochrome P450 as Autoantigens.
R.J. Edwards, B.P. Murray, and A.R. Boobis, Antipeptide Antibodies in Studies of Cytochromes P450IA.
A. Parkinson and B. Gemzik, Production and Purification of Antibodies against Rat Liver P450 Enzymes.
Selective Measurement of Protein and mRNA Expression:
D.J. Waxman, Rat Hepatic P450IIA and P450IIC Subfamily Expression Using Catalytic, Immunochemical, and Molecular Probes.
M. Negishi, B. Burkhart, and K. Aida, Expression of Genes within Mouse IIA and IID Subfamilies: Simultaneous Measurement of Homologous P450 mRNAs.
J.P. Hardwick, CYP4A Subfamily: Functional Analysis by Immunohistochemistry and in Situ Hybridization.
R.H.~Tukey and S. Okino, Quantitation of Related Gene Products by Nuclear Run-On and Northern Blot Analysis.
C. Hassett, R. Ramsden, and C.J. Omiecinski, Isolating Cytochrome P450 cDNA and Genomic Clones: Library Screening with Synthetic DNA Oligomers.
Regulatory Paradigms:
J.A. Koch and D.J. Waxman, P450 Phosphorylation in Isolated Hepatocytes and in Vivo.
M.A. Correia, Cytochrome P450 Turnover.
J.B. Schenkman, Induction of Diabetes and Evaluation of Diabetic State on P450 Expression.
Cell Culture Systems:
D. Li, E.G. Schuetz, and P.S. Guzelian, Hepatocyte Culture in Study of P450 Regulation.
M. Daujat, L. Pichard, I. Fabre, T. Pineau, G. Fabre, C. Bonfils, and P. Maurel, Induction Protocols Cytochromes P450IIIA for in Vivo and in Primary Cultures~of Animal and Human Hepatocytes.
G.G. Gibson and B.G. Lake, Induction Protocols for Cytochrome P450IVA Subfamily in Animals and Primary Hepatocyte Cultures.
D.R. Koop, R.M. Laethem, A.L. Goldner, and J.G. Douglas, Cytochrome P450 Expression and Metabolism Isolated Rabbit Renal Epithelium.
P.J. Hornsby and J.M. McAllister, Culturing Steroidogenic Cells.
O. Hankinson, Selection for and against Cells Possessing Cytochrome P450IA1-Dependent Aryl Hydrocarbon Hydroxylase Activity.
Transcriptional Regulation:
E.S. Shen, C.J. Elferink, and J.P. Whitlock, Jr. , Use of Gel Retardation to Analyze Protein-DNA Interactions Upstream of CYPIA1 Gene.
T.H. Rushmore and C.B. Pickett, Xenobiotic Responsive Elements Controlling Inducible Expression by Planar~Aromatic Compounds and Phenolic Antioxidants.
Enzyme Assays:
A.D. Rodrigues and R.A. Prough, Induction of Cytochromes P450IA1 and P450IA2 and Measurement of Catalytic Activities.
R.T. Okita, J.E. Clark, J.R. Okita, and B.S.S. Masters, ~gu and (~gu-1)-Hydroxylation of Eicosanoids and Fatty Acids by High-Performance Liquid Chromatography.
J.H. Capdevila, E. Dishman, A. Karara, and J.R. Falck, Cytochrome P450 Arachidonic Acid Epoxygenase: Stereochemical Characterization of Epoxyeicosatrienoic Acids.
M.P. Arlotto, J.M. Trent, and R.W. Estabrook, Measurement of Steroid Hydroxylation Reactions by HPLC as an Indicator of P450 Idenity and Function.
D.J. Waxman, P450-Catalyzed Steroid Hydroxylation: Assay and Product Identification by Thin-Layer Chromatography.
~E.D. Lephart and E.R. Simpson, Assay of Aromatase Activity.
J.Y.L. Chiang, Reversed-Phase HPLC Assay of Cholesterol 7~ga-Hydroxylase.
J.L. Napoli, C.A. Martin, and R.L. Horst, Induction, Inhibition, and Analysis of Vitamin D Metabolism in Cultured Cells.
M.E. McManus and R.A. McKinnon, Measurement of Cytochrome P450 Activation of Xenobiotics Using Ames/Salmonella Test.
T. Kronbach, Bufuralol, Dextromethorphan, and Debrisoquine as Prototype Substrates for Human P450IID6.
P.B. Watkins, Breath Tests as Noninvasive Assays of P450s.
L.A. Waskell, J.L. Vigne, and G. Vergeres, Site of Action of Substrates Requiring Cytochrome b5 for Oxidation by Cytochrome P450.
Design and Characterization of Inhibitors:
P.R. Ortiz de Montellano, Mechanism-Based~Inactivation of Cytochrome P450: Isolation and Characterization of N-Alkyl Heme Adducts.
J.R. Halpert and J.C. Stevens, Dihalomethyl Compounds as Mechanism-Based Inactivators of Cytochromes P450.
L.E. Vickery, Active Site-Directed Inhibitors of Steroid Hydroxylase Cytochromes P450.
M.R. Franklin, Cytochrome P450 Metabolic or Intermediate Complexes from Macrolide Antibiotics and Related Compounds.
Isolation Procedures:
J.L. Raucy and J.M. Lasker, Isolation of P450 Enzymes from Human Liver.
R.M. Shayiq, S. Addya, and N.G. Avadhani, Constitutive and Inducible Forms of Cytochrome P450 from Hepatic Mitochondria.
C.S. Yang, C.J. Patten, H. Ishizaki, and J.-S.H. Yoo, Induction, Purification, and Characterization of Cytochrome P450IIE.
X. Ding~and M.J. Coon, Purification and Characterization of Cytochromes P450 in Rabbit Nasal Microsomes.
J.A. Peterson and J.-Y. Lu, Bacterial Cytochromes P450: Isolation and Identification.
Characterization of Extrahepatic Forms of P450:
R.M. Philpot, Characterization of Cytochrome P450 in Extrahepatic Tissues.
M. Warner, R. Ahlgren, P.G. Zaphiropoulos, S.-I. Hayashi, and J.-A. Gustafsson, Identification and Localization of Cytochrones P450 Expressed in Brain.
M. Str~adomstedt, P.G. Zaphiropoulos, and J.-A. Gustafsson, Identification of Cytochrome P450 in Extrahepatic Tissues by Cross-Hybridization of Oligonucleotides and cDNAs.
H.W. Strobel, D.K. Hammond, T.B. White, and J.W. White, Identification and Localization of Cytochromes~P450 in Gut.
Each chapter includes references.
Author Index.
Subject Index.
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pt. B ISBN 9780121821739
内容説明
The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. More than 270 volumes have been published (all of them still in print) and much of the material is relevant even today--truly an essential publication for researchers in all fields of life sciences.
目次
Heterologous Expression of P450s: H.J. Barnes, Maximizing Expression of Eukaryotic Cytochrome P450s in Escherichia coli. C.W. Fisher, M.S. Shet, and R.W. Estabrook, Construction of Plasmids and Expression in Escherichia coli of Enzymatically Active Fusion Proteins Containing the Heme-Domain of a P450 Linked to NADPH-P450 Reductase. S.J. Pernecky and M.J. Coon, N-Terminal Modifications That Alter P450 Membrane Targeting and Function. F.P. Guengerich, M.V. Martin, Z. Guo, and Y.-J. Chun, Purification of Recombinant P450s from Bacteria. R.W. Estabrook, K.M. Faulkner, M.S. Shet, and C.W. Fisher, Application of Electrochemistry for P450-Catalyzed Reactions. D. Pompon, B. Louerat, A. Bronine, and P. Urban, Yeast Expression of Animal and Plant 450s in Optimized Redox Environments. U. Scheller, T. Juretzek, and W.-H. Schunck, Generation of the Cytosolic Domain of Microsomal P450 52A3 after High-Level Expression in Saccharomyces cerevisiae. S. Lecoeur, J.-C. Gautier, C. Belloc, A. Gauffre, and P. Beaune, Use of Heterologous Expression Systems to Study Autoimmune Drug-Induced Hepatitis. C.A. Lee, T.A. Kost, and C. Serabjit-Singh, Recombinant Baculovirus Strategy for Coexpression of Functional Human Cytochrome P450 and P450 Reductase. Enzyme Assays: H.K. Kroemer, S. Botsch, G. Heinkele, and M. Schick, In Vitro Assessment of Various Cytochromes P450 and Glucuronosyltransferases Using the Antiarrhythmic Propafenone as a Probe Drug. P.J. Wedlund and G.R. Wilkinson, In Vivo and in Vitro Measurement of CYP2C19 Activity. D. Lucas, J.-F. Menez, and F. Berthou, Chlorzoxazone: An in Vitro and in Vivo Substrate Probe for Liver CYP2E1. B.K.Tang and W. Kalow, Assays for CYP1A2 by Testing in Vivo Metabolism of Caffeine in Humans. D.J. Birkett, T. Andersson, and J.O. Miners, Assays of Omeprazole Metabolism as a Substrate Probe for Human CYP Isoforms. J.O. Miners and D.J.Birkett, Use of Tolbutamide as a Substrate Probe for Human Hepatic Cytochrome P450 2C9. T. Walle, Assays of CYP2C8- and CYP3A4-Mediated Metabolism of Taxol in Vivo and in Vitro. D. Kupfer and S.S. Dehal, Tamoxifen Metabolism by MicrosomalCytochrome P450 and Flavin-Containing Monooxygenase. E. Tanaka and Y. Funae, Trimethadione: Metabolism and Assessment of Hepatic Drug-Oxidizing Capacity. K. Groen and D.D. Breimer, Antipyrine, Theophylline, and Hexobarbital as in Vivo P450 Drug Probes. K. Brosen, E. Skjelbo, and K.K. Nielson, Imipramine: A Model Drug for P450 Research. A.D. Rodrigues, Measurement of Human Liver Microsomal Cytochrome P450 2D6 Activity Using [O-methyl14C]Dextromethorphan as Substrate. Determination of Genotype: A.K. Daly, V.M. Steen, K.S. Fairbrother, and J.R. Idle, CYP2D6 Multiallelism. J.A. Goldstein and J. Blaisdell, Genetic Tests which Identify the Principle Defects in CYP2C19 Responsible for the Polymorphismin Mephenytoin Metabolism. V. Nedelcheva, I. Persson, and M. Ingelman-Sundberg, Genetic Polymorphism of Human Cytochrome P450 2E1. K. Kawajiri, J. Watanabe, and S.-i. Hayashi, Identification of Allelic Variants of Human CYP1A1 Gene. Plant P450s: S.-M.C. Lau and D.P. OKeefe, Analysis of Herbicide Metabolism by Monocot Microsomal Cytochrome P450. J. Hefner and R. Croteau, Microsome Preparation from Woody Plant Tissues. A.R. Brash and W. Song, Detection, Assay, and Isolation of Allene Oxide Synthase. F. Durst, I.Benveniste, M. Schalk, and D. Werck-Reichhart, Cinnamic Acid Hydroxylase Activity in Plant Microsomes. B.A. Halkier, O. Sibbesen, and B.L. Moller, Isolation of Plant and Recombinant CYP79. T.A. Holton and D.R. Lester, Cloning of Novel Cytochrome P450 Gene Sequences via PCR Amplification. Insect P450s: J.G. Scott, Preparation of Microsomes from Insects and Purification of CYP6D1 from House Flies. T.J. Sliter, S. Sudhivoraseth, and J.L. McCarthy, Quantification of Ecdysteroid Biosynthesis during Short Term Organ Culture. M.J. Synder, J.A. Scott, J.F. Anderson, and R. Feyereisen, Sampling P450 Diversity by Cloning PCR Products Obtained with Degenerate Primers. Analysis of P450 Structure: S.E. Graham-Lorence and J.A. Peterson, Structural Alignments of P450s and Extrapolations to the Unknown. K.R. Korzekwa and J.P. Jones, Predicting the Rates and Regioselectivity of Reactions Mediated by the P450 Superfamily. J.J. de Voss and P.R. Ortiz de Montellano, Substrate Docking Algorithms and the Prediction of Substrate Specificity. M.D. Paulsen, J.I. Manchester, and R.L. Ornstein, Using Molecular Modeling and Molecular Dynamics Simulation to Predict P450 Oxidation Products. T.L. Poulos, Approaches to Crystallizing P450s. J.-J.P. Kim, D.L. Roberts, S. Djordjevic, M. Wang, T. Shea, and B.S.S. Masters, Crystallization Studies of NADPH-Cytochrome P450 Reductase. Regulation: E.T. Morgan, M. Nikolova-Karakashian, J.-q. Chen, and A.H. Merrill, Jr., Sphingolipid-Dependent Signaling in Regulation of Cytochrome P450 Expression. S.C. Strom, L.A. Pisarov, K. Dorko, M.T. Thompson, J.D. Schuetz, and E.G. Schuetz, Use of Human Hepatocytes to Study P450 Gene Induction. M.J. Fasco, E. Treanor, and L. Kaminsky, Cytochrome P450 mRNA Induction: Quantitation by RNA-PCR Using Capillary Electrophoresis. P.M. Fernandez-Salguero and F.J. Gonzalez, Targeted Disruption of Specific Cytochromes P450 and Xenobiotic Receptor Genes. Author Index. Subject Index.
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pt. C ISBN 9780121822606
内容説明
The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today truly an essential publication for researchers in all fields of life sciences.
目次
Section I: Human Genomics and Genetics
Mining Databases for Cytochrome P450 Genes.
Sequence Alignments, Variabilities, and Vagaries.
Human CYP Allele Database: Submission Criteria Procedures and Objectives.
Fine-Scale Mapping of CYP Gene Clusters: An Example from Human CYP4 Family.
Detection of Single Nucleotide Polymorphisms in CYP2B6 Gene.
Genotyping Human Cytochrome P450 1B1 Variants.
Genotyping Human CYP2A Variants.
Section II: Structure and Mechanism
Purification and Crystallization of N-Terminally Truncated Forms of Microsomal Cytochrome P450 2C5.
Molecular Replacement in P450 Crystal Structure Determinations.
Optical Biosensor and Scanning Probe Microscopy Studies of Cytochrome P450 Interactions with Redox Partners and Phospholipid Layers.
Cryoradiolysis for the Study of P450 Reaction Intermediates.
Analyzing Binding of N-Terminal Truncated, Microsomal Cytochrome P450s to Membranes.
Sensitizer-Linked Substrates and Ligands: Ruthenium Probes of Cytochrome P450 Structure and Mechanism.
Combining Pharmacophore and Protein Modeling to Predict CYP450 Inhibitors and Substrates.
High Pressure: A New Tool to Study P450 Structure and Function.
Section III: Regulation of Expression
Use of in Vitro PXR Assays to Assess CYP3A4 Induction Potential of Drug Candidates.
Analysis of CYP mRNA Expression by Branched DNA Technology.
Ligand-Induced Coactivator Recruitment to PPAR-Characterized by Fluorescence Resonance Energy Transfer.
Fluorescence-Based Ligand Binding Assays for PPARs.
Developing Toxicologically Predictive Gene Sets Using cDNA Microarrays and Bayesian Classification.
Direct Expression of Fluorescent Protein-Tagged Nuclear Receptor CAR in Mouse Liver.
Application of Fluorescent Differential Display and PPAR-Null Mice to Analyze PPAR Target Genes.
Histological and Metabolism Analysis of P450 Expression in the Brain.
Proteomic Analysis of Rodent Hepatic Responses to Peroxisome Proliferators.
Section IV: Metabolism
Kinetic Analysis for Multiple Substrate Interaction at the Active Site of Cytochrome P450.
Design and Application of Fluorometric Assays for Human Cytochrome P450 Inhibition.
Automated Quantitative and Qualitative Analysis of Metabolic Stability: A Process for Compound Selection during Drug Discovery.
Characterization of Covalent Adducts to Intact Cytochrome P450s by Mass Spectrometry.
Mutagenesis Testing Based on Bacterial Expression of Human P450s.
Use of Long-Term Cultures of Human Hepatocytes to Study Cytochrome P450 Gene Expression..
Polarized Cell Cultures for Integrated Studies of Drug Metabolism and Transport.
Section V: Invertebrate P450s
Use of Methylotropic Yeast Pichia pastoris for Expression of Cytochromes P450.
Partial Recoding of P450 and P450 Reductase cDNAs for Improved Expression in Yeast and Plants.
Selective Covalent Labeling with Radiolabeled Suicide Substrates for Isolating P450s.
Cloning of cDNAs Encoding P450s in Flavonoid/Isoflavonoid Pathway from Elicited Leguminous Cell Cultures.
Selected Cell Cultures and Induction Methods for Cloning and Assaying Cytochromes P450s in Alkaloid Pathways.
Isolation and Functional Characterization of Cytochrome P450s in Gibberellin Biosynthesis Pathway.
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