Biosensors
著者
書誌事項
Biosensors
（Techniques and instrumentation in analytical chemistry, v. 11）
Elsevier, 1992
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Biosensoren
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注記
Rev. translation of: Biosensoren
Includes bibliographical references and index
内容説明・目次
内容説明
Biosensors combine the unique properties of biological systems to selectively recognize and convert molecules with the benefits of physicochemical sensor technology, such as high sensitivity, simplicity of operation and mass production, and modern electronics. Consequently, their development is closely related to progress in two branches of high technology - biotechnology and microelectronics. This book not only presents the state-of-the-art of biosensor research and development to the specialist, but also introduces the layman to the fundamentals of the subject. The relevant features of physicochemical transducer elements as well as biochemical recognition molecules (enzymes, antibodies, receptors) are outlined. Biochemical and biotechnological aspects of biomolecule immobilization and the interplay of biochemical reactions and mass transfer processes are comprehensively treated with regard to their impact on successful sensor design. Examples of immobilization methods are described in detail. The employment of coupled enzyme reactions, higher integrated biocatalytic systems (cell organelles, microbes, tissue sections) and immunocomponents in biosensors is covered extensively. Optical, thermometric, piezoelectric and particularly electrochemical biosensors for more than 100 analytes are presented, including immunosensors. The relative merits and limits of biosensors are discussed using several examples of their application in clinical chemistry, bioprocess control and environmental monitoring. Finally, the application of biosensors in medicine, biotechnology, food industry and environmental control is discussed, including commercialization and problems to be addressed in future research.
目次
1. Introduction. 2. Physicochemical, Biochemical and Technological Fundamentals of Biosensors. Biosensors as Functional Analogs of Chemoreceptors. Structure and Function of Transducers. Biochemical Fundamentals. Immobilization of the Receptor Component in Biosensors. Mathematical Modeling of Amperometric Enzyme Electrodes. 3. Metabolism Sensors. Monoenzyme Sensors. Biosensors Using Coupled Enzyme Reactions. Biosensors Using Higher Integrated Biocatalysts. 4. Affinity Biosensors. Affinity Sensors Using Low-Molecular Weight Ligands. Affinity Sensors Based on Proteins and Enzymes. Immunosensors. Biosensors Using Intact Biological Receptors. 5. Application of Biosensors. General Aspects. Biosensors for Clinical Chemistry. Continuous Patient Monitoring and Implantable Sensors. Food Analysis, Bioprocess Control and Environmental Monitoring. 6. Perspectives - Combination of Biotechnology and Microelectronics in Biosensors. List of Abbreviations and Symbols. References. Subject Index.
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