In situ hybridization : a practical approach


In situ hybridization : a practical approach

edited by D.G. Wilkinson

(The practical approach series, 109)

IRL Press, c1992

  • : pbk

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Includes bibliographical references and index



The detection of specific nucleic acid sequences by in situ hybridization has many important applications in modern biology. In situ detection of viral DNA sequences is enabling the diagnosis of human diseases, the detection of specific sequences on chromosomes is crucial for genome analysis, and in fields such as developmental biology and neurobiology, the investigation of gene expression by in situ hybridization to mRNA has become a key technique. "In Situ Hybridization - A Practical Approach" is a comprehensive practical guide to the uses of this major technique. Step-by-step protocols are presented for the preparation of cloned DNA, RNA and oligonucleotide probes, with both radioactive and non-radioactive labels, and their hybridization to mRNA, viral DNA, and chromosomal DNA. Methods are provided for the analysis of cells, tissue sections, and whole embryos. In addition, techniques are presented for carrying out in situ hybridization in combination with the immunochemical detection of protein, and for using it at the electron microscopic level. This book is aimed at any researcher wishing to exploit the many widesprad applications of this important technique.


  • The theory and practice of in situ hybridization, D.G. Wilkinson
  • in situ hybridization to cellular RNA with radiolabelled RNA probes, L.M. Angerer and R.C. Angerer
  • in situ hybridization with oligodeoxyribonucleotide probes, W. Scott Young III
  • in situ hybridization with biotinylated probes, P. Emson and M.J. Gait
  • whole mount in situ hybridization in Drosophila, D. Tautz et al
  • whole mount in situ hybridization of vertebrate embryos, D.G. Wilkinson
  • simultaneous detection of cellular RNA and proteins, M. Brahic and S. Ozden
  • in situ hybridization at the electron microscope level, M. Binder
  • detection of virus in infected human tissue by in situ hybridization, F.A. Lewis and M. Wells
  • in situ hybridization to chromosomes with biotinylated probes, E. Viegas-Pequignot.

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  • ISBN
    • 0199633282
    • 0199633274
  • LCCN
  • 出版国コード
  • タイトル言語コード
  • 本文言語コード
  • 出版地
    Oxford ; Tokyo
  • ページ数/冊数
    xvii, 163 p.
  • 大きさ
    24 cm
  • 分類
  • 件名
  • 親書誌ID