Animal cell culture : a practical approach
著者
書誌事項
Animal cell culture : a practical approach
（The practical approach series）
IRL Press at Oxford University Press, c1992
2nd ed
- : hbk
- : pbk
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注記
Includes bibliographical references and index
内容説明・目次
内容説明
This new edition takes account of the progress that has been made in the field since the publication of the first edition in 1986. Although the basic principles remain the same, significant advances have been made in areas such as serum-free media, scale-up, and flow cytometry. As these techniques have developed as tools for the cell biologist, their availability to the non-specialist has also increased dramatically. Use of the tetrazolium salt MTT as a colorimetric indicator of viability has made a considerable impact on cytotoxicity assay, and DNA fingerprinting has revolutionized the identification of individual cell strains. These, and other developments in the techniques described have made this new edition essential. The emphasis remains on presenting techniques in a readily accessible form, with detailed protocols given throughout. This volume should be of use to researchers involved in both biological research and the commercial exploitation of animal cell culture.
目次
- R.I. Freshney: Introduction to basic principles
- H.R. Maurer: Towards serum-free, chemically defined media for mammalian cell culture
- B. Griffiths: Scaling-up of animal cell cultures
- R.J. Hay: Cell line preservation and characterization
- D. Conkie: Separation of viable cells by centrifugal elutriation
- J.V. Watson & E. Erba: Flow cytometry
- I. Lasnitzki: Organ culture
- A.P. Wilson: Cytotoxicity and viability assays
- S.M. Lang, A.H. Wyllie, & D. Conkie: In situ hybridization
- Index.
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