Fundamentals
著者
書誌事項
Fundamentals
（Methods of immunological analysis / editor-in-chief, René F. Masseyeff ; editors, Winfried H. Albert, and Norman A. Staines, v. 1）
VCH Verlagsgesellschaft , VCH Publishers, c1993
- : Weinheim
- : New York
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注記
Includes bibliographical references and index
内容説明・目次
内容説明
This first volume in a set of 12 on immunological analysis covers the basic fundamentals of the subject which are required before an understanding of its various applications can be grasped. Aimed at biochemists, immunologists, molecular biologists and pharmacologists, this book provides a complete up-to-date compilation of all methods of immunological analysis, presented in a standard format to allow optimal access to the information. The first three volumes in the series supply a practice-oriented introduction to immunological analysis. The remaining eight volumes provide laboratory sheets of immunoanalytical techniques in various fields of the life sciences, chemistry, and medicine. One or more reliable methods are described for each analyte or class of analytes, typifying alternative approaches. In this way, users will find specific methods for widely-measured analytes that can be implemented without modification. Moreover, they can extract soundly-based principles and experimental techniques on which to construct immunoassays for new or less familiar analytes.
Topics covered include immunoglobulins, complement components, auto-antibodies, tumour-related antigens, and various hormones and blood proteins. Each chapter is designed to give scientists enough information to reproduce the technique described quickly and accurately.
目次
- Part 1 Introduction: definition of immunological analysis, relationships with other disciplines
- metrological aspects of immonological analysis. Part 2 Essentials of immunology for the analyst: the immune system and the immune response
- antigens, immunogens, haptens
- antobody response and clonality
- the complement system
- molecular basis of antigen-antobody reactions. Part 3 Principles of immunoassays: classification of methods
- precipitation and agglutination methods
- methods using labelled reagents
- labels and their measurement
- non-separation methods (selected examples)
- separation (distribution) methods
- methods employing complement
- enzyme-immunoassays in molecular biology. Part 4 Implementation of immunoassays: design and equipment of immunoassays
- optimization and validation of methods
- limitations and drawbacks
- data analysis
- evaluation - comparison of methods. Appendix: symbols, quantities, units and constants.
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