Cell-cell interactions : a practical approach
著者
書誌事項
Cell-cell interactions : a practical approach
(The practical approach series)
IRL Press at Oxford University Press, c1992
- :pbk
- :hbk
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注記
Includes bibliographical references and index
内容説明・目次
- 巻冊次
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:pbk ISBN 9780199633180
内容説明
This book provides a wealth of practical information relating to this area of intense research in cell biology. It describes the methods essential to the identification and characterization of the molecules that mediate cellular interactions in a variety of systems. Invaluable to a wide range of biological scientists, the book includes methods for preparing relevant probes, analysing adhesive and junctional interactions in multicellular organizations, determining
which structural features of a relevant molecule are functionally important, and for assaying the consequences of cell-cell interactions in cell culture.
目次
- Assays of cell adhesion, S. Hofman
- generation and characterization of function-blocking anti-cell adhesion molecule antibodies, Ann Acheson and Warren Gallin
- expression cloning - transient expression and rescue from mammalian cells, Alejandro Aroffo
- functional analysis of uvomorulin by site-directed mutagenesis and gene transfer into eukaryotic cells, Jorg Stappert and Rolf Kemler
- integrin mutagenesis, A. Reszka and A. Horwitz
- isolation of intercellular junctions, Elliot L. Hertzberg et al
- the "Xenopus" oocyte cell-cell channel assay for functional analysis of gap junction proteins, Gerhard Dahl
- patch-clamp analysis of gap junction currents, R.D. Veenstra and P.R. Brink
- biochemical approaches for analyzing "de novo" assembly of epithelial junctional components, Manijeh Pasdar
- biochemical methods for studying supramolecular complexes involving cell adhesion molecules, integral membrane proteins, and the cytoskeleton, W. James Nelson et al
- electrophysiological assessment of epithelia, Karl J. Karnaky, Jr
- compartmented culture analysis of nerve growth, Robert B. Campenot.
- 巻冊次
-
:hbk ISBN 9780199633197
内容説明
The type of communication between a cell and its immediate environment defines, to a large extent, the phenotype of that cell; it also determines the structure and function of the tissues and organs of which the cell is a part. This book describes the methods essential to the identification and characterization of the molecules that mediate cellular interactions in a variety of systems. Included are methods for preparing relevant probes, analyzing adhesive and junctional interactions in multicellular organizations, determining which structural features of a relevant molecule are functionally important, and for assaying the consequences of cell-cell interactions in cell culture. "Cell-cell Culture" aims to provide practical information relating to this area of cell biology. This book should therefore be useful to a range of biological scientists wishing to investigate the mechanisms and consequences of cellular interactions in their system of choice.
目次
- Assays of cell adhesion, S. Hofman
- generation and characterization of function-blocking anti-cell adhesion molecule antibodies, Ann Acheson and Warren Gallin
- expression cloning - transient expression and rescue from mammalian cells, Alejandro Aroffo
- functional analysis of uvomorulin by site-directed mutagenesis and gene transfer into eukaryotic cells, Jorg Stappert and Rolf Kemler
- integrin mutagenesis, A. Reszka and A. Horwitz
- isolation of intercellular junctions, Elliot L. Hertzberg et al
- the "Xenopus" oocyte cell-cell channel assay for functional analysis of gap junction proteins, Gerhard Dahl
- patch-clamp analysis of gap junction currents, R.D. Veenstra and P.R. Brink
- biochemical approaches for analyzing "de novo" assembly of epithelial junctional components, Manijeh Pasdar
- biochemical methods for studying supramolecular complexes involving cell adhesion molecules, integral membrane proteins, and the cytoskeleton, W. James Nelson et al
- electrophysiological assessment of epithelia, Karl J. Karnaky, Jr
- compartmented culture analysis of nerve growth, Robert B. Campenot.
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