Lipopolysaccharides, Separation and analysis, Glycosylated polymers
著者
書誌事項
Lipopolysaccharides, Separation and analysis, Glycosylated polymers
（Methods in carbohydrate chemistry / editors, Roy L. Whistler, M. L. Wolfrom ; assistant editors, James N. BeMiller, F. Shafizadeh, v. 9）
Wiley, c1993
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注記
Editors, James N. BeMiller, Roy L. Whistler; associate editor, Derek H. Shaw
Includes bibliographies and indexes
内容説明・目次
内容説明
A practical bench-side reference for carbohydrate chemistry
Methods in Carbohydrate Chemistry: Lipopolysaccharides, Separation and Analysis, Glycosylated Polymers (Volume 9) presents proven techniques for working with carbohydrates in the lab. Topic experts contribute insights and protocols for membrane isolation and purification, glycoprotein synthesis, and carbohydrate immobilization, with detailed guidance on chromatographic, chemical, enzymatic, and physical methods of separation and analysis. Helpful flow charts provide easy bench-side reference, while proven methods allow for predictable, repeatable results. Anyone who encounters carbohydrates in the lab will find value in this clear, practical reference.
目次
Partial table of contents:
LIPOPOLYSACCHARIDES.
Isolation and Purification of Lipopolysaccharides (K. Johnson).
Determination of the Structure of the Carbohydrate Backbone of Lipid A (S. Hase & E. Rietschel).
SEPARATION AND ANALYSIS.
Identification and Characterization of Saccharides by GLC Separation and MS Analysis of Their Peracetylated Aldononitrile (PAAN) and Ketooxime (PAKO) Derivatives (F. Seymour).
Determination of Pyruvic Acid in Complex Polysaccharides (M. Duckworth & J. Madden).
NATURAL AND SYNTHETIC GLYCOPROTEINS.
Attachment of Sugars to Proteins by Amidination (C. Stowell & Y. Lee).
IMMOBILIZATION OF CARBOHYDRATES.
Immobilization of Carbohydrates on Poly(Acrylamide) Gels I: Poly(Acrylamide) Gels Copolymerized with Active Esters (R. Schnaar, et al.).
Index.
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