Essential developmental biology : a practical approach
著者
書誌事項
Essential developmental biology : a practical approach
（The practical approach series）
IRL Press, c1993
- : hbk
- : pbk
大学図書館所蔵 件 / 全33件
-
該当する所蔵館はありません
- すべての絞り込み条件を解除する
注記
Includes bibliographical references and index
内容説明・目次
- 巻冊次
-
: pbk ISBN 9780199634224
内容説明
Developmental biology is a newly-developing subject that is attracting much interest because of exciting findings being made using a combination of classical embryo manipulation with more modern techniques. This book brings together for the first time, and in comprehensive, easy-to-follow protocols, practical instructions for all of the main techniques, from traditional embryology to cellular and molecular methods. It includes complete reprints of all the stage
tables in common use for the main laboratory species. It will become an essential addition to laboratory benches of those using any technique as applied to embryos, cells, or tissues. It will also help teachers to design practical classes suitable for undergraduate and graduate courses.
目次
- PART I: BASIC TECHNIQUES: OBTAINING AND HANDLING EMBRYOS
- 1. Drosophila embryos
- 2. Nematode embryos
- 3. Embryos and larvae of invertebrate deuterostomes
- 4. Zebrafish embryos
- 5. Xenopus
- 6. Avian embryos
- 7. Post-implantation mammalian embryos
- 8. Simple tips for photomicrography or embryos
- PART II: EXPERIMENTAL EMBRYOLOGY
- 9. Fate maps and cell lineage analysis
- 10. Microinjection into Drosophila embryos
- 11. Cell transplantation in Xenopus
- 12. Transplantation in avian embryos
- 13. Chick limb buds
- 14. Nuclear transplantation
- 15. Laser ablation of cells
- PART III: CELLULAR TECHNIQUES
- 16. Studying cell movements in vivo by time-lapse video microscopy
- 17. Induction assays in Xenopus
- 18. Culture of avian neural crest cells
- 19. ES cells and gene targeting
- 20. Introduction of genes using retroviral vectors
- PART IV: MOLECULAR TECHNIQUES
- 21. Immunocytochemistry of embryonic material
- 22. RNase protection assays
- 23. cDNA library construction
- 24. Analysis of gene expression by RT-PCR
- 25. Cloning genes using PCR
- 26. In situ hybridisation
- Appendices
- I: Stage tables
- a. Westerfield for zebrafish embryos
- b. Nieukoop & Faber for Xenopus laevis
- c. Eyal-Giladi & Kochav for early chick
- d. Hamburger & Hamilton for later chick
- II: Suppliers of specialist items
- Index
- 巻冊次
-
: hbk ISBN 9780199634231
内容説明
In recent years, developmental biology has undergone a revolution. The introduction of new techniques of molecular and cell biology, couples with those designed by classical embryologists, has provided a new approach to the study of mechanisms that control early animal development. As new techniques continue to be developed and refined, there is now an immediate need for a book that brings together approaches from many different disciplines as they apply to embryos. This volume brings together easy-to-follow protocols and practical instructions for all of the main techniques in classical embryo manipulation, from traditional embryology to cellular and molecular methods. It includes reprints of all the stage tables in common use for the main laboratory species. It continues with practical instruction on techniques for ebryological manipulations such as fate mapping, cell lineage analysis, ablation and transplantation, together with molecular methods for constructing cDNA libraries, cloning genes, analyzing their expression and disrupting their action during development. Each chapter is written by authorities in the field.
An addition to the book are reprints of the most commonly-used staging tables for zebrafish, amphibian and chick embryos. This book should provide a guide for those who wish to broaden their approach to cover different animal species, or to move across the boundaries between molecular, cellular and embryological strategies.
目次
- Part 1 Basic techniques - obtaining and handling embryos: drosophila embryos, Zandy Forbes and Philip Ingham
- nematode embryos, Lois G. Edgar and William B. Wood
- embryos and larvae of invertebrate deuterostomes, Nicholas D. Holland and Linda Z. Holland
- zebrafish embryos, Rachel M. Warga
- Xenopus, Ariel Ruis I. Altaba
- avian embryos, Claudio D. Stern
- post-implantation of mammalian embryos, Gillian M. Morriss-Kay
- simple tips for photomicrography of embryos, Claudio D. Stern. Part 2 Experimental embryology: fate maps and cell lineage analysis, Ariel Ruiz I. Altaba et al
- micro-injection into drosophila embryos, Philip Ingham and Zandy Forbes
- cell transplantation in xenopus, Ariel Ruiz I. Altaba
- transplantation in avian embryos, Claudio D. Stern
- chick limb buds, Cheryll tickle
- nuclear transplantation, John B. Gordon
- laser ablation of cells, Judith Eisen et al. Part 3 Cellular techniques: studying cell movements in vivo by time-lapse video microscopy, Peter V. Thorogood and Dee Amanze
- induction assays in Xenopus, Ariel Ruiz I. Altaba
- culture of avian neural crest cells, Elisabeth Dupin and Nicole M. Le Douarin
- embryonic stem cells and gene targeting, Elizabeth J. Robertson and Gail R. Martin
- introduction of genes using retroviral vectors, Jack Price. Part 4 Molecular techniques: immonocytochemistry of embryonic material, Claudio D. Stern
- RNAase protection assays, Ariel Ruiz I. Altaba
- cDNA library construction, Akira Kakizuka et al
- analysis of gene expression by reverse transcription polymerase chain reaction, Peter Koopman
- cloning genes using the polymerase chain reaction, Peter W.H. Holland
- in situ hybridization, David G. Wilkinson. Appendices: stage tables
- suppliers of specialist items.
「Nielsen BookData」 より