Protein phosphorylation : a practical approach
著者
書誌事項
Protein phosphorylation : a practical approach
（The practical approach series）
Oxford University Press, c1993
- : hbk
- : pbk
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注記
Includes bibliographical references and index
内容説明・目次
- 巻冊次
-
: pbk ISBN 9780199633050
内容説明
Reversible phosphorylation is one of the major mechanisms by which protein activity is controlled in all eukaryotic cells. Networks of protein kinases and phosphatases, which catalyze phosphorylation and dephosphorylation respectively, are responsible for switching between different pathways of cell division, development or differentiation in response to hormones or growth factors. This book provides a comprehensive description of current methods for studying protein phosphorylation and the protein kinases and phosphatases which catalyze it. It includes detailed protocols for studying phosphorylation in intact cells, approaches using enzyme inhibitors, purification of serine/threonine-specific protein kinases/phosphatases, analysis of kinase/phosphatase specificity and cloning relevant cDNAs. It should be of interest to researchers in the fields of intracellular and intercellular signalling, including biochemists, molecular biologists and pharmacologists.
目次
- 1. Study of protein phosphorylation in intact cells
- 2. Phosphopeptide mapping and phosphoamino acid analysis on cellulose thin layer plates
- 3. Analysis of sites phosphorylated in vivo and in vitro
- 4. Use of protein (serine/threonine) kinase activators and inhibitors to study protein phosphorylation in intact cells
- 5. Use of protein phosphatase inhibitors in intact cells
- 6. Purification and assay of mammalian protein (serine/theonine) kinases
- 7. Purification of protein (tyrosine) kinases
- 8. Homology-based approaches for identifying cDNAs that encode eukaryotic protein (serine-threonine) and protein (tyrosine) kinases
- 9. Assay and purification of protein (serine/threonine) phosphatases
- 10. Characterization of protein (tyrosine) phosphatases
- 11. Identification of novel protein (serine/threonine) phosphatase genes by saccharomyces cerevisiae, drosophila melanogaster, and man by the polymerase chain reaction
- 12. Studies of protein kinase/phosphatase specificity using synthetic peptides
- 巻冊次
-
: hbk ISBN 9780199633067
内容説明
Reversible phosphorylation is one of the major mechanisms by which protein activity is controlled in all eukaryotic cells. Networks of protein kinases and phosphatases, which catalyze phosphorylation and dephosphorylation respectively, are responsible for switching between different pathways of cell division, development or differentiation in response to hormones or growth factors. This book provides a comprehensive description of current methods for studying protein phosphorylation and the protein kinases and phosphatases which catalyze it. It includes detailed protocols for studying phosphorylation in intact cells, approaches using enzyme inhibitors, purification of serine/threonine-specific protein kinases/phosphatases, analysis of kinase/phosphatase specificity and cloning relevant cDNAs. It should be of interest to researchers in the fields of intracellular and intercellular signalling, including biochemists, molecular biologists and pharmacologists.
目次
- Study of protein phosphorylation in intact cells, J.C. Garrison
- phosphopeptide mapping and phosphoamino acid analysis on cellulose thin-layer plates, P. van der Geer et al
- analysis of sites phosphorylated in vivo and in vitro, D.G. Hardie et al
- use of protein (serine/threonine) kinase activators and inhibitors to study protein phosphorylation in intact cells, D.G. Hardie
- use of protein phosphatase inhibitors in intact cells, Hiroyashi Hidaka and Ryoji Kobayashi
- purification and assay of mammalian protein (serine/threonine) kinases, Y. Wang and P.J. Roach
- purification of protein (tyrosine) kinases, A.A. Stewart
- homology-based approaches for identifying cDNAs that encode eukaryotic protein (serine/threonine) and protein (tyrosine) kinases, S. Hanks and M.A. Lawton
- assay and purification of protein (serine/threonine) phosphatases, C. MacKintosh
- characterization of protein (tyrosine) phosphatases, N.K. Tonks
- identification of novel protein (serine/threonine) phosphatase genes by saccharomyces cerevisiae, drosophila melanogaster and man by the polymerase chain reaction, Mao Xiang Chen and P.T.W. Cohen
- studies of protein kinase/phosphatase specificity using synthetic peptides, R.B. Pearson et al.
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