Culture of animal cells : a manual of basic technique
Author(s)
Bibliographic Information
Culture of animal cells : a manual of basic technique
Wiley-Liss, c1994
3rd ed
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Note
Bibliography: p. 437-469
Includes index
Description and Table of Contents
Description
In this edition, the author provides a comprehensive synthesis of proven methods for the culture and experimental manipulation of animal cells. Containing step-by-step laboratory procedures, detailed instructions for reagent preparation, and information on latest specialized equipment and supplies, this laboratory manual includes over 350 new references, 13 new protocols plus recent methods and expanded protocols in areas of new technology such as antibody staining and cytotoxicity assay.
Table of Contents
- Biology of the Cultured Cell
- Design and Layout of the Laboratory
- Equipping the Laboratory
- Aseptic Technique
- Laboratory Safety and Biohazards
- The Culture Environment: Substrate, Gas Phase, Medium and Temperature
- Preparation and Sterilization
- Disaggregation of the Tissue and Primary Culture
- Maintenance of the Culture: Cell Lines
- Cloning and Selection of Cell Strains
- Physical Methods of Cell Separation
- Characterization
- Induction of Differentiation
- The Transformed Phenotype
- Contamination
- Instability, Variation and Preservation
- Quantitation and Experimental Design
- Measurement of Viability and Cytotoxicity
- Culture of Specific Cell Types
- Culture of Tumour Tissue
- Three-Dimensional Culture
- Specialized Techniques.
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