PCR in situ hybridization : protocols and applications

書誌事項

PCR in situ hybridization : protocols and applications

Gerard J. Nuovo

Raven Press, c1994

2nd ed

大学図書館所蔵 件 / 38

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注記

Includes bibliographical references and index

内容説明・目次

内容説明

Highlights of this second edition contain more detailed information on the hot start PCR technique. Expanded discussion of the cell-localizing ability of in situ hybridization includes complete consideration of oligoprobes. Other topics featured are: the detection of such viruses as the human papillomavirus and the human immunodeficiency virus-1; RT in situ PCR, including step-by-step protocols and applications; and an update on the detection of hepatitis C and measles. Dr Nuovo is the co-author of "Genital Papillomaviruses and Related Neoplasms".

目次

  • The basics of molecular biology
  • the nonspecific pathways of PCR
  • in situ hybridization
  • PCR in situ hybridization
  • a starter's guide to in situ PCR - the start up protocol
  • reverse transcription in situ PCR
  • applications of PCR in situ hybridization - human papillomavirus, human immunodeficiency virus-1
  • materials for molecular biological analyses.

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