Expression systems
Author(s)
Bibliographic Information
Expression systems
（The practical approach series, v. 149 . DNA cloning : a practical approach ; 2）
IRL Press, c1995
2nd ed
- : hbk
- : pbk
Available at / 41 libraries
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Okayama University Institute of Plant Science and Resources Branch Library
: hbk165||361S205000208131*
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National Institutes of Natural Sciences Okazaki Library and Information Center図
: pbk463/DN/29218384213
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Note
Includes bibliographical references and indexes
Description and Table of Contents
- Volume
-
: pbk ISBN 9780199634781
Description
Expression systems are in widespread use for cloning specific genes, for synthesising the encoded proteins for structural and functional analysis, and for large-scale preparation of current use with background information and advice on the merits of each, step-by-step practical protocols, troubleshooting, and details of the latest applications.
These two books, the first of four volumes are thoroughly revised and up-dated versions of the original ones published in the Practical Approach series. Together they will offer a complete guide to the major techniques required by modern molecularbiology laboratories.
Table of Contents
- 1. Screening expression libraries with antibody and protein probes
- 2. Expression of foreign proteins in E.coli using plasmid vectors and purification of specific polyclonal antibodies
- 3. Purification of over-produced proteins from E.coli cells
- 4. Production of monoclonal antibodies against proteins expressed in E.coli
- 5. Expression of cloned genes in yeast
- 6. Intraction trap cloning with yeast
- 7. The baculovirus expression system
- Volume
-
: hbk ISBN 9780199634798
Description
Expression systems are in widespread use for cloning specific genes, for synthesizing the encoded proteins for structural and functional analysis, and for large-scale preparation of proteins of commercial interest. Part of a four-volume set on DNA cloning, this book covers the main expression systems in current use. It provides background information and advice on the merits of each, step-by-step practical protocols, and details of the latest applications.
Table of Contents
- Screening expression libraries with antibody and protein probes
- Expression of foreign proteins in E.coli using plasmid vectors and purification of specific polyclonal antibodies
- Purification of over-produced proteins from E.coli cells
- Production of monoclonal antibodies against proteins expressed in E.coli
- Expression of cloned genes in yeast
- Intraction trap cloning with yeast
- The baculovirus expression system.
by "Nielsen BookData"
