Expression systems
著者
書誌事項
Expression systems
（The practical approach series, v. 149 . DNA cloning : a practical approach ; 2）
IRL Press, c1995
2nd ed
- : hbk
- : pbk
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注記
Includes bibliographical references and indexes
内容説明・目次
- 巻冊次
-
: pbk ISBN 9780199634781
内容説明
Expression systems are in widespread use for cloning specific genes, for synthesising the encoded proteins for structural and functional analysis, and for large-scale preparation of current use with background information and advice on the merits of each, step-by-step practical protocols, troubleshooting, and details of the latest applications.
These two books, the first of four volumes are thoroughly revised and up-dated versions of the original ones published in the Practical Approach series. Together they will offer a complete guide to the major techniques required by modern molecularbiology laboratories.
目次
- 1. Screening expression libraries with antibody and protein probes
- 2. Expression of foreign proteins in E.coli using plasmid vectors and purification of specific polyclonal antibodies
- 3. Purification of over-produced proteins from E.coli cells
- 4. Production of monoclonal antibodies against proteins expressed in E.coli
- 5. Expression of cloned genes in yeast
- 6. Intraction trap cloning with yeast
- 7. The baculovirus expression system
- 巻冊次
-
: hbk ISBN 9780199634798
内容説明
Expression systems are in widespread use for cloning specific genes, for synthesizing the encoded proteins for structural and functional analysis, and for large-scale preparation of proteins of commercial interest. Part of a four-volume set on DNA cloning, this book covers the main expression systems in current use. It provides background information and advice on the merits of each, step-by-step practical protocols, and details of the latest applications.
目次
- Screening expression libraries with antibody and protein probes
- Expression of foreign proteins in E.coli using plasmid vectors and purification of specific polyclonal antibodies
- Purification of over-produced proteins from E.coli cells
- Production of monoclonal antibodies against proteins expressed in E.coli
- Expression of cloned genes in yeast
- Intraction trap cloning with yeast
- The baculovirus expression system.
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