Environmental microbiology : a laboratory manual
著者
書誌事項
Environmental microbiology : a laboratory manual
Academic Press, c1995
- : acid-free paper
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注記
Includes bibliographical references
内容説明・目次
内容説明
Environmental Microbiology: A Laboratory Manual is designed to meet the diverse requirements of upper division and graduate-level laboratory sessions in environmental microbiology. The experiments introduce students to the activities of various organisms and the analyses used to study them. The book is organized into three thematic sections: Soil Microbiology, Water Microbiology, and Environmental Biotechnology. The first section includes experiments on soil as a habitat for microorganisms, and introduces the main types of soil microorganisms, how they interact with the soil, and the techniques used in their analysis. Experiments in the second section cover assays of microbial pathogens -- bacteria, viruses, and protozoan parasites -- used in food and water quality control as well as an exercise in applied bioremediation of contaminants in water. The final section on biotechnology includes applications of the polymerase chain reaction (PCR) for the detection of bacteria and the use of enrichment cultures and a computer-based, physiological test bank to isolate and identify a bacterium useful in bioremediation.
Designed for maximum versatility and ease of use for both the student and instructor, each experiment is self-contained and includes theoretical, practical, and pedagogical material.
目次
- Part 1 Basic soil microbiology experiments: moisture content detemination
- contact slide assay
- filamentous fungi
- bacteria and actinomycetes
- algae
- oxidation of sulphur in soil
- dehydrogenase activity of soils
- nitrification and denitrification. Part 2 Basic water mcirobiology: coliform detection by MPN
- membrane filter technique
- detection of bacteriophages
- assimilable organic carbon
- new methods for the detection of fecal coliforms
- detection of coliforms in water food and on surfaces
- detection of enteric viruses in water
- detection of waterborne parasites. Part 3 Environmental biotehcnology: biodegradation of phenol compounds
- enrichment culture and BIOLOG
- toxicity testing by use of a bioassay
- disinfection methodology through UV light
- molecular detection of microbial pathogens via polymerase chain reaction
- fingerprinting via arbitrarily primed polymerase chain raction (AP-PCR)
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