Gene probes

著者

• Hames, B. D.
• Higgins, S. J.

書誌事項

Gene probes

edited by B. D. Hames and S. J. Higgins

（The practical approach series, 161-162）

IRL Press at Oxford University Press, c1995

• 1 : hbk
• 1 : pbk
• 2 : hbk
• 2 : pbk

注記

Includes bibliographical references

収録内容

• 1. Preparation
• 2. Applications

内容説明・目次

巻冊次

1 : pbk ISBN 9780199634002

内容説明

The preparation of gene probes is a core application in molecular biology creating essential tools for genetic research. This volume together with its forthcoming companion volume, Gene Probes 2, provides a timely and important resource for all researchers in this field. Gene Probes 1 focuses on the wide range of techniques now available for the purification, synthesis, labelling, detection, and quantitation of probes, including antibody probes. Gene Probes 2 will concentrate on describing techniques for specific research goals. Contents List: Strategies for using gene probes; Preparation of nucleic acids for use as probes; DNA radiolabelling and detection; Non-radioactive labelling of DNA probes; Preparation of oligodeoxynucleotide probes; Probe preparation by PCR; Synthesis of labelled riboprobes; Antibody probes

目次

• Introduction
• 1. Strategies for using gene probes
• 2. Preparation of nucleic acids for use as probes
• 3. DNA radiolabelling and detection
• 4. Non-radioactive labelling of DNA probes
• 5. Preparation of synthetic oligodeoxynucleotide probes
• 6. Probe preparation of PCR
• 7. Synthesis of labelled riboprobes
• 8. Antibody probes
• Appendix 1
• Appendix 2

巻冊次

1 : hbk ISBN 9780199634019

内容説明

The preparation of gene probes is a core application in molecular biology, creating essential tools for genetic research. A companion volume, Gene Probes 2 , covers applications of the probes. The two books are timely additions to the series, as this is a progressive field which continues to grow in importance. This book is intended for this book will be of interest to researchers in a wide variety of fields e.g. molecular biology, molecular genetics, pathology, and developmental biology.

目次

Introduction. 1.: Strategies for using gene probes. 2.: Preparation of nucleic acids for use as probes. 3.: DNA radiolabelling and detection. 4.: Non-radioactive labelling of DNA probes. 5.: Preparation of synthetic oligodeoxynucleotide probes. 6.: Probe preparation of PCR. 7.: Synthesis of labelled riboprobes. 8.: Antibody probes. Appendix 1. Appendix 2

巻冊次

2 : pbk ISBN 9780199634149

内容説明

Gene probes find a wide variety of uses in molecular biology, and are essential tools for genetic research. This volume describes some of their many applications, from the study of nucleic acids, through blotting techniques and gene targeting, to analysis of human genetic mutations. A Companion volume, Genes Probes 1, covers preparation of the probes. The two books are timely additions to the series, as this is a progressive field which continues to grow in importance.

目次

• 1. Hybridization strategy
• 2. Screening recombinant DNA libraries by hybridization and amplification
• 3. Screening human recombinant libraries with nucleic acid probes
• 4. Antibody screening of bacterial expression systems
• 5. Isolation of cDNA clones by direct expression in mammalian cells
• 6. Genomic mapping and gene structure analysis
• 7. Analysis of gene expression by blotting techniques
• 8. In situ hybridization of nucleic acid probes to cellular RNA
• 9. Applications of chromosomal in situ hybridization
• 10. Analysis of gene expression analysis by PCR: a quantitative approach
• 11. Analysis of protein expression by immunocytochemistry
• 12. Gene targeting in mammalian cells
• 13. Directed mutagenesis and mutant analysis
• 14. Human gene mutational analysis
• Appendix 1
• Appendix 2

巻冊次

2 : hbk ISBN 9780199634156

内容説明

Gene probes find a wide variety of uses in molecular biology, and are essential tools for genetic research. This volume describes some of their many applications, from the study of nucleic acids, through blotting techniques and gene targeting, to the analysis of human genetic mutations. A companion volume covers the preparation of these probes. The volumes aim to contribute to a growing field of research.

目次

1.: Hybridization strategy. 2.: Screening recombinant DNA libraries by hybridization and amplification. 3: Screening human recombinant libraries with nucleic acid probes. 4.: Antibody screening of bacterial expression systems. 5.: Isolation of cDNA clones by direct expression in mammalian cells. 6.: Genomic mapping and gene structure analysis. 7.: Analysis of gene expression by blotting techniques. 8.: In situ hybridization of nucleic acid probes to cellular RNA. 9.: Applications of chromosomal in situ hybridization. 10.: Analysis of gene expression analysis by PCR: a quantitative approach. 11.: Analysis of protein expression by immunocytochemistry. 12.: Gene targeting in mammalian cells. 13.: Directed mutagenesis and mutant analysis. 14.: Human gene mutational analysis. Appendix 1. Appendix 2

「Nielsen BookData」 より

詳細情報

• NII書誌ID(NCID): BA26600404
• ISBN:
  • 0199634017
  • 0199634009
  • 0199634157
  • 0199634149
• LCCN: 95018740
• 出版国コード: uk
• タイトル言語コード: eng
• 本文言語コード: eng
• 出版地: Oxford ; New York
• ページ数/冊数: 2 vols
• 大きさ: 24 cm
• 分類:
  • LCC : QP624.5.D73
  • DC20 : 574.87/322
• 件名:
  • LCSH : DNA probes
• 親書誌ID:
  • BA00227768