Lipid analysis
Author(s)
Bibliographic Information
Lipid analysis
(The Introduction to biotechniques series)
BIOS Scientific Publishers, 1996
Available at 11 libraries
  Aomori
  Iwate
  Miyagi
  Akita
  Yamagata
  Fukushima
  Ibaraki
  Tochigi
  Gunma
  Saitama
  Chiba
  Tokyo
  Kanagawa
  Niigata
  Toyama
  Ishikawa
  Fukui
  Yamanashi
  Nagano
  Gifu
  Shizuoka
  Aichi
  Mie
  Shiga
  Kyoto
  Osaka
  Hyogo
  Nara
  Wakayama
  Tottori
  Shimane
  Okayama
  Hiroshima
  Yamaguchi
  Tokushima
  Kagawa
  Ehime
  Kochi
  Fukuoka
  Saga
  Nagasaki
  Kumamoto
  Oita
  Miyazaki
  Kagoshima
  Okinawa
  Korea
  China
  Thailand
  United Kingdom
  Germany
  Switzerland
  France
  Belgium
  Netherlands
  Sweden
  Norway
  United States of America
Note
Includes bibliographical references (p. 171) and index
Description and Table of Contents
Description
There is increasing recognition of the diversity and biological importance of lipids. Lipid modifications of other biological molecules are now also the subject of intense research activity. This 'user-friendly' introduction describes the techniques curre
Table of Contents
Abbreviations -- Preface -- 1. Introduction -- 2. Basic Techniques -- Extraction methods -- Lipid binding in tissues -- Solvents for lipid extraction -- Some precautions -- Storage of lipid extracts -- Saponification -- Chromatographic procedures -- General considerations -- Partition chromatography -- Adsorption chromatography -- Ion exchange chromatography -- Complexing chromatography -- Spectrometric methods -- UV-visible spectrometry -- IR spectroscopy -- NMR spectroscopy -- Mass spectrometry -- Radioisotopic methods -- Choice of radioisotope -- Autoradiography -- Scintillation counting -- Gas ionization detection -- Gamma counting -- Radioisotopes in lipid studies -- RIA and related techniques -- Immunochemical methods -- Antibodies to lipids -- Hemagglutination in detection and assay -- Immunostaining and TLC -- Immunoradiometric assays -- Enzyme-linked immunosorbent assays -- Analytical methods involving enzymes -- Phospholipases -- Stereospecific analysis of triacylglycerol -- Enzymes in quantifying lipids -- Presentation of lipids in aqueous systems -- Care and safety during the analysis of lipids in biological materials -- General considerations -- Chemical hazards -- Fire hazards -- Radioisotope hazards -- Biological hazards -- Other hazards -- Personal preparedness and protection -- References -- 3. Hydrocarbons -- Biological significance -- Structures -- Detection -- Isolation and purification -- Quantitation -- References -- 4. Alcohols, Phenols, Aldehydes, Ketones and Quinones -- Alcohols and phenols -- General and biological significance -- Structures -- Detection -- Isolation and purification -- Quantitation -- Aldehydes and ketones -- General and biological significance -- Structures -- Detection -- Isolation and purification -- Quantitation -- Quinones -- General and biological significance -- Structures -- Detection -- Isolation and purification -- Quantitation -- References -- 5. Fatty Acids and Prostaglandins -- Fatty acids -- Types of fatty acids found naturally -- Separation methods for fatty acids -- GLC of fatty acids -- HPLC of fatty acids -- Prostaglandins and leukotrienes (eicosanoids) -- Analysis of eicosanoids -- References -- 6. Esters -- Acylglycerols -- Separation of acylglycerols -- Analysis of acylglycerols -- Fatty acids of acylglycerols -- Wax esters -- Alkylglycerols -- Analytical methods for alkylglycerols -- References -- 7. Phospholipids, Sulfolipids and Related Compounds -- Phospholipids with amino-groups -- Hydrolytic procedures in analysis -- Estimation of phosphate -- Separation of phospholipids -- Phosphoinositides -- Phosphoinositide and inositol phosphate metabolism -- Separation of the phosphoinositides -- Receptor-linked phosphoinositide changes -- Separation and estimation of inositol phosphates -- Estimation of diacylglycerol -- Phosphatidylinositol glycans -- Detection of PI glycans -- Phosphonolipids -- Separation and analysis of phosphonolipids -- Alkylether phospholipids -- Analysis of alkylether phospholipids -- Sulfolipids -- Lipid sulfates -- Sulfonolipids -- Analytical methods -- References -- 8. Glycolipids -- Glycosphingolipids -- Biological significance -- Structures -- Detection -- Isolation and purification -- Quantitation -- Polyisoprenyl phosphosugars -- Biological significance -- Structures -- Detection -- Isolation and purification -- Quantitation -- Glycosylglycerides and related compounds -- Biological significance -- Structures -- Detection -- Isolation and purification -- Quantitation -- Phosphoglycolipids, lipoteichoic acids and related compounds -- Biological significance -- Structures -- Detection, isolation, preparation and quantitation -- References -- 9. Lipoproteins -- Plasma lipoproteins -- Structures and biological significance -- Detection -- Isolation and purification -- Quantitation -- Fatty acyl proteins and prenyl proteins -- Biological significance -- Structures -- Detection -- Isolation and purification -- Quantitation -- References -- Appendices -- Appendix A: Glossary -- Appendix B: Suppliers -- Appendix C: Further reading -- Index.
by "Nielsen BookData"