PCR protocols for emerging infectious diseases : a supplement to Diagnostic molecular microbiology: principles and applications

著者

書誌事項

PCR protocols for emerging infectious diseases : a supplement to Diagnostic molecular microbiology: principles and applications

edited by David H. Persing

ASM Press, c1996

タイトル別名

Diagnostic molecular microbiology: principles and applications

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注記

Includes bibliographical references and index

内容説明・目次

内容説明

The subject of emerging infectious diseases has recently captured international attention with the identification of previously unrecognized human pathogens and the arrival of multiple drug resistant forms of more conventional agents. Examples of recently recognized or emerging infectious diseases include hepatitis C, hantavirus pulmonary syndrome, ebola virus infection, human ehrlichiosis, Lyme disease, babesiosis, cat-scratch disease and multiple drug resistant tuberculosis. PCR Applications for Emerging Pathogens is a supplement to the ASM publication, Diagnostic Molecular Microbiology, which was published in 1993. This supplement is designed to provide a technological update for clinical microbiologists, and to enable practical application of some of the most powerful new technologies. Together with Diagnostic Molecular Microbiology this new manual will provide all the theoretical and practical information you need to operate a state of the art facility for doing molecular diagnosis of infectious diseases.

目次

  • Part I. Molecular Diagnostic Applications for Emerging Infections
  • Molecular Methods for Pathogen Identification
  • Genotype Detection of Antimicrobial Resistance
  • Nucleic Acid Sequencing Studies of Microbial Pathogens: Insights into Epidemiology, Virulence, Drug Resistance, and Diversity
  • Part II. Selected Protocols for Emerging Infections
  • Detection of Sin Nombre Hantavirus by Reverse Transcriptase-PCR
  • Genus- and Species-level Identification of Ehrlichia Species by Using PCR and Sequencing
  • Detection of the Agent of Human Granulocytic Ehrlichiosis by PCR
  • Detection of Rifampin Resistance Mutations in Mycobacterium tuberculosis by Dideoxy Fingerprinting
  • PCR-Heteroduplex Detection of Rifampin-Resistant Mycobacterium tuberculosis
  • Detection of Mycobacteria Resistance to Streptomycin and Clarithromycin
  • Detection of Fluroquinolone Resistance Mutations in Mycobacterium tuberculosis
  • PCR-RFLP Detection of Point Mutations in the Catalase-Peroxidase Gene (katG) of Mycobacterium tuberculosis Associated with Isoniazid Resistance
  • Detection of Enterococcal Vancomycin Resistance by Multiplex PCR
  • Genetic Detection of the Streptococcal Pyrogenic Exotoxin Gene by PCR
  • Detection of Cryptosporium Parvum by PCR
  • Detection of Cyclospora

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