Antibody applications : essential techniques


Antibody applications : essential techniques

Peter J. Delves

(Essential techniques series)

Wiley , Published in association with BIOS Scientific Publishers, c1995

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Includes bibliographical references and index



This convenient lab manual gives quick and easy reference to key protocols and practical tips needed to undertake antibody applications. Thirty-five protocols are listed including a troubleshooting guide and tips to indicate hazards, good points to stop methods, key conditions, and reagent disposal.


  • INTRODUCTORY MATERIAL: Characterization of Antibodies
  • Labelling of Antibodies
  • Applications of Antibodies
  • PROTOCOLS: Antibody Concentration by Optical Density
  • Assessment of Purity of Antibody Preparation using SDS-PAGE
  • Measurement of Affinity using Friguet Method
  • Labelling of Antibodies with Biotin
  • Labelling of Antibodies with 125I
  • Labelling of Antibodies with Fluorochrome
  • Labelling of Antibodies with Enzyme
  • Direct ELISA
  • Sandwich ELISA
  • Competitive ELISA for Antigen Detection
  • Competitive ELISA for Epitope Mapping
  • Fluid Phase Radioimmunoassay
  • Cell Surface Immunofluorescence
  • Intracellular Immunofluorescence on Cytospines
  • Intracellular Immunofluorescence on Cell Suspensions
  • Immunofluorescence on Tissue Sections
  • Enzyme-Linked Immunohistology
  • Immunoaffinity Chromatography using Columns
  • Batch Immunoaffinity Chromatography
  • Immunoprecipitation of Fluid Phase Antigen
  • Immunoprecipitation of Cellular Antigens
  • Ouchterlony Double Immunodiffusion
  • Single Radial Immunodiffusion.

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