Culture and biochemical analysis of cells
著者
書誌事項
Culture and biochemical analysis of cells
（Cells : a laboratory manual / David L. Spector, Robert D. Goldman, Leslie A. Leinwand, v. 1）
Cold Spring Harbor Laboratory Press, c1998
- : cloth
- : comb
並立書誌 全2件
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注記
Includes bibliographical references
内容説明・目次
- 巻冊次
-
: comb ISBN 9780879695217
内容説明
Having identified a gene product, how do you determine what it does? This manual seeks to answer this question, with a collection of strategies and techniques for the study of cellular components. It contains step-by-step protocols, ranging widely from basic culture of vertebrate and non-vertebrate cells, through advanced detection methods using immunological, cytochemical and molecular in-situ approaches, and imaging techniques such as confocal and deconvolution microscopy.
目次
- Volume 1 Culture and biochemical analysis of cells: cell culture metabolic labelling of cells/organisms
- subcellular fractionation
- protein separation and identification
- systems for protein expression
- antibodies as tools in cell biology. Volume 2 Light microscopy and cell strucure: preparation of live cells for observation by light microscopy
- preparation of macromolecules and introduction into cells
- light and epifluorescence microscopy
- confocal microscopy and deconvolution techniques. Volume 3 Subcellular localization of genes and their products: visualization of proteins, organelles and gene expression
- in situ hybridization
- electron microscopy
- immunoelectron microscopy.
- 巻冊次
-
: cloth ISBN 9780879695224
内容説明
Having identified a gene product, how do you determine what it does? This three-volume study seeks to answer this question, and provides strategies and techniques for the study of cellular components. It contains step-by-step protocols of proven reliability, ranging widely from basic culture of vertebrate and non-vertebrate cells, metabolic labelling, purification of organelles, introduction and visualization of molecules in live cells, through advanced detection methods using cytochemical, immunological and in situ hybridization approaches, and imaging techniques such as fluorescence, confocal and electron microscopy.
目次
- Volume 1 Culture and biochemical analysis of cells: cell culture and analysis
- metabolic labelling and protein modification
- subcellular fractionation
- protein identification and analysis
- protein expression and interaction
- antibodies as tools in cell biology. Volume 2 Light microscopy and cell structure: observation of live cells and cellular dynamics
- preparation of macromolecules and introduction into cells
- light and epifluorescence microscopy
- confocal and multiphoton microscopy and deconvolution. Volume 3 Subcellular localization of genes and their products: visualization of organelles, proteins and gene expression
- in situ hybridization
- electron microscopy.
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