Subcellular localization of genes and their products
Author(s)
Bibliographic Information
Subcellular localization of genes and their products
（Cells : a laboratory manual / David L. Spector, Robert D. Goldman, Leslie A. Leinwand, v. 3）
Cold Spring Harbor Laboratory Press, c1998
- : cloth
- : comb
Related Bibliography 2 items
Available at / 100 libraries
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Note
Includes bibliographical references and index
Description and Table of Contents
- Volume
-
: comb ISBN 9780879695217
Description
Having identified a gene product, how do you determine what it does? This manual seeks to answer this question, with a collection of strategies and techniques for the study of cellular components. It contains step-by-step protocols, ranging widely from basic culture of vertebrate and non-vertebrate cells, through advanced detection methods using immunological, cytochemical and molecular in-situ approaches, and imaging techniques such as confocal and deconvolution microscopy.
Table of Contents
- Volume 1 Culture and biochemical analysis of cells: cell culture metabolic labelling of cells/organisms
- subcellular fractionation
- protein separation and identification
- systems for protein expression
- antibodies as tools in cell biology. Volume 2 Light microscopy and cell strucure: preparation of live cells for observation by light microscopy
- preparation of macromolecules and introduction into cells
- light and epifluorescence microscopy
- confocal microscopy and deconvolution techniques. Volume 3 Subcellular localization of genes and their products: visualization of proteins, organelles and gene expression
- in situ hybridization
- electron microscopy
- immunoelectron microscopy.
- Volume
-
: cloth ISBN 9780879695224
Description
Having identified a gene product, how do you determine what it does? This three-volume study seeks to answer this question, and provides strategies and techniques for the study of cellular components. It contains step-by-step protocols of proven reliability, ranging widely from basic culture of vertebrate and non-vertebrate cells, metabolic labelling, purification of organelles, introduction and visualization of molecules in live cells, through advanced detection methods using cytochemical, immunological and in situ hybridization approaches, and imaging techniques such as fluorescence, confocal and electron microscopy.
Table of Contents
- Volume 1 Culture and biochemical analysis of cells: cell culture and analysis
- metabolic labelling and protein modification
- subcellular fractionation
- protein identification and analysis
- protein expression and interaction
- antibodies as tools in cell biology. Volume 2 Light microscopy and cell structure: observation of live cells and cellular dynamics
- preparation of macromolecules and introduction into cells
- light and epifluorescence microscopy
- confocal and multiphoton microscopy and deconvolution. Volume 3 Subcellular localization of genes and their products: visualization of organelles, proteins and gene expression
- in situ hybridization
- electron microscopy.
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