Chromatin : a practical approach
著者
書誌事項
Chromatin : a practical approach
（The practical approach series）
IRL Press at Oxford University Press, 1998
- :hbk
- :pbk
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注記
Includes bibliographical references and index
内容説明・目次
- 巻冊次
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:pbk ISBN 9780199635986
内容説明
Chromatin comprises the complex of nucleic acids and proteins that make up the chromosomes, and regulation of its structure is increasingly recognized as an important factor in gene expression. Researchers from related fields needing an introduction to the methodology of chromatin should find this book useful. It should also be of interest to experienced researchers.
目次
- 1. Isolation and fractionation of chromatin and linker histones
- 2. Extraction and separation of core histones and non-histone chromosomal proteins
- 3. Mapping chromatin structure in Drosophila
- 4. In vivo footprint and chromatin analysis by LMPCR
- 5. Analysis of nuclear scaffold attachment regions
- 6. Electron microscopy of chromatin
- 7. Counting nucleosome cores on circular DNA using topoisomerase I
- 8. In vitro reconstitution of chromatin and analysis of nucleosome positioning
- 9. Transcriptional and structural analysis of chromatin assembled in vivo
- 10. An assembly of chromatin and nuclear structures in Xenopus egg extracts
- Appendix: List of Suppliers
- Index
- 巻冊次
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:hbk ISBN 9780199635993
内容説明
Chromatin comprises the complex of nucleic acids and proteins that make up the chromosomes, and regulation of its structure is increasingly recognized as an important factor in gene expression. Researchers from related fields needing an introduction to the methodology of chromatin should find this book useful. It should also be of interest to experienced researchers.
目次
1: Isolation and fractionation of chromatin and linker histones. 2: Extraction and separation of core histones and non-histone chromosomal proteins. 3: Mapping chromatin structure in Drosophila. 4: In vivo footprint and chromatin analysis by LMPCR. 5: Analysis of nuclear scaffold attachment regions. 6: Electron microscopy of chromatin. 7: Counting nucleosome cores on circular DNA using topoisomerase I. 8: In vitro reconstitution of chromatin and analysis of nucleosome positioning. 9: Transcriptional and structural analysis of chromatin assembled in vivo. 10: An assembly of chromatin and nuclear structures in Xenopus egg extracts. Appendix: List of Suppliers. Index
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