Biomedical electron microscopy : illustrated methods and interpretations

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書誌事項

Biomedical electron microscopy : illustrated methods and interpretations

Arvid B. Maunsbach, Björn A. Afzelius

Academic Press, c1999

大学図書館所蔵 件 / 13

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注記

Includes bibliographical references and index

内容説明・目次

内容説明

This comprehensive reference illustrates optimal preparation methods in biological electron microscopy compared with common methodological problems. Not only will the basic methodologies of transmission electron microscopy like fixation, microtomy, and microscopy be presented, but the authors also endeavor to illustrate more specialized techniques such as negative staining, autoradiography, cytochemistry, immunoelectron microscopy, and computer-assisted image analysis.

目次

Micrograph InterpretationFixativesFixative VehicleFixative ApplicationDehydration and EmbeddingFreezing and Freeze-SubstitutionSupport FilmsUltramicrotomySection-StainingMicroscopyImage RecordingPhotographic and Digital PrintingNegative StainingAutoradiographyCytochemistryImmunocytochemistryFreeze-Fracturing and ShadowingSampling and QuantitationImage Processing3D ReconstructionsAppendix: Practical Methods

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