Protein phosphorylation : a practical approach
著者
書誌事項
Protein phosphorylation : a practical approach
(The practical approach series, 211)
Oxford University Press, c1999
2nd ed
- : hbk
- : pbk
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注記
Includes bibliographical references and index
内容説明・目次
内容説明
Reversible phosphorylation is one of the major mechanisms of controlling protein activity in all eukaryotic cells. This new edition of Protein Phosphorylation: A Practical Approach provides a comprehensive description of current methods used to study protein phosphorylation and the kinases and phosphatases which catalyse it. It includes protocols for studying phosphorylation in intact cells; analysis of signal transduction pathways, kinase specificity, and
kinase interactions; assay and purification of kinases and phosphatases; and identification of substrates. Also covered are cloning and expression protocols and advice on the crystallization of kinases and phosphatases. Protein Phosphorylation: A Practical Approach 2e will therefore be of great value to any
researcher investigating aspects of reversible protein phosphrylation.
目次
- Preface
- 1. Study of protein phosphorylation in intact cells
- 2. Analysis of signal transduction pathways using protein kinase inhibitors and activators
- 3. Analysis of signal transduction pathways using proteinserine/threonine phosphatase inhibitors
- 4. Analysis of signal transduction pathways using molecular biological manipulations
- 5. Phosphopeptide mapping and phosphoamino acid analysis on cellulose thin-layer plates
- 6. Phosphorylation site analysis by mass spectrometry
- 7. Assay and purification of protein (serine/threonine) phosphatases
- 8. [chapter title missing]
- 9. Identification of substrates for protein-tyrosine phosphatases
- 10. Assay and purification of protein-serine/threonine kinases
- 11. Assay of protein kinases and phosphatases using specific peptide substrates
- 12. Cloning and expression of cDNAs encoding protein kinase subunits
- 13. Interaction cloning of protein kinase partners
- 14. Identifying protein kinase substrates by expression screening with solid-phase phosphorylation
- 15. Analysis of protein kinase interactions using the two-hybrid method
- 16. Analysis of protein kinase interactions using biomolecular interaction analysis
- 17. Analysis of protein kinase specificity using orientated peptide libraries
- 18. Crystallization of protein kinases and phosphatases
- Index
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