Arabidopsis : a practical approach
著者
書誌事項
Arabidopsis : a practical approach
（The practical approach series, 223）
Oxford University Press, c2000
- : hbk
- : pbk
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注記
Includes bibliographical references and index
内容説明・目次
- 巻冊次
-
: pbk ISBN 9780199635641
内容説明
Arabidopsis has long been acknowledged as the 'Botanical Drosophila' with its small genome, low levels of repetitive DNA, small size and fast generation time it is an ideal molecular genetic tool for the analysis of development in higher plants. Arabidopsis: A Practical Approach provides an introduction to most of the key techniques required for the use of Arabidopsis as an experimental system. It gives a basic introduction to
the optimal growth conditions and genetic resources available for Arabidopsis, how this material should be handled, maintained and used. Individual chapters describe strategies for the identification, mapping (using multi-marker lines and recombinant inbreds), and characterisation of different mutants by microscopy, molecular
cytogenetics and gene expression analysis. Different cloning strategies, using transposons, T-DNA and map position are described in detail. Sequencing of the Arabidopsis genome will be completed in 2000 and bioinformatics are of key importance; the tools that are available and where they can be found on the Web are presented.
目次
- Growth, maintenance and use of Arabidopsis genetic resources
- Preservation and handling of stock centre clones
- Genetic mapping using recombinant inbred lines
- ARABIDOPSIS MUTANT CHARACTERISATION: MICROSCOPY, MAPPING AND GENE EXPRESSION ANALYSIS
- Classical and molecular cytogenetics of Arabidopsis
- Tissue culture, transformation and transient gene expression in Arabidopsis
- Transposon and T-DNA mutagenesis
- Map based cloning in Arabidopsis
- YACs, BACs, cosmids and nucleotide sequences
- Web-based bioinformatic tools for Arabidopsis researchers
- 巻冊次
-
: hbk ISBN 9780199635658
内容説明
Arabidopsis has long been acknowledged as the 'Botanical Drosophila' with its small genome, low levels of repetitive DNA, small size and fast generation time it is an ideal molecular genetic tool for the analysis of development in higher plants. Arabidopsis: A Practical Approach provides an introduction to most of the key techniques required for the use of Arabidopsis as an experimental system. It gives a basic introduction to the optimal
growth conditions and genetic resources available for Arabidopsis, how this material should be handled, maintained and used. Individual chapters describe strategies for the identification, mapping (using multi-marker lines and recombinant inbreds), and characterisation of different mutants by microscopy, molecular
cytogenetics and gene expression analysis. Different cloning strategies, using transposons, T-DNA and map position are described in detail. Sequencing of the Arabidopsis genome will be completed in 2000 and bioinformatics are of key importance; the tools that are available and where they can be found on the Web are presented.
目次
- Growth, maintenance and use of Arabidopsis genetic resources
- Preservation and handling of stock centre clones
- Genetic mapping using recombinant inbred lines
- ARABIDOPSIS MUTANT CHARACTERISATION: MICROSCOPY, MAPPING AND GENE EXPRESSION ANALYSIS
- Classical and molecular cytogenetics of Arabidopsis
- Tissue culture, transformation and transient gene expression in Arabidopsis
- Transposon and T-DNA mutagenesis
- Map based cloning in Arabidopsis
- YACs, BACs, cosmids and nucleotide sequences
- Web-based bioinformatic tools for Arabidopsis researchers
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