Humason's animal tissue techniques

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Bibliographic Information

Humason's animal tissue techniques

Janice K. Presnell and Martin P. Schreibman

Johns Hopkins University Press, c1997

5th ed

Available at  / 1 libraries

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Includes bibliographical references and index

Humason's Animal Tissue Techniques is the fifth edition of Animal Tissue Techniques by Gretchen L. Humason

Description and Table of Contents

Description

Gretchen L. Humason began her study of zoology and histological procedures in the 1920s. Passing away in 1991, Humason left behind over half a century of research study and instruction. Now, true to the traditions of Humason and based on her philosophies, expertise and recommendations, Janice K. Presnell and Martin Schreibman bring us the newly revised fifth edition of Humason's literary contributions to the theory and operation of histotechnology. Drawing on her life's work, "Humason's Animal Tissue Techniques" presents basic and standard tissue study procedures as well as specialized histological techniques. Designed as a guide to the principles and methods involved in histotechnology, the text begins with an overview of the history of microtechnology and laboratory safety. It then proceeds to review basic procedures such as fixation, dehydration and staining methods for specific tissue elements. Also discussed are special procedures and considerations: histochemistry, immunohistochemical methods, microwave histology, sex chromatin and chromosomes, preparation of invertebrate whole mounts and sections, and much more.

Table of Contents

  • Part 1 Historical perspectives: overview of the development of microtechnology. Part 2 Laboratory safety: practical aspects of safety
  • special provisions for the handicapped. Part 3 Basic procedures: fixation
  • dehydration - preparation for embedding
  • clearing
  • infiltrating
  • embedding - paraffin method
  • microtomes and microtome knives
  • paraffin sectioning and mounting
  • the microscope
  • stains and staining action
  • mounting and staining procedures
  • haematoxylin staining. Part 4 Specific staining methods: staining connective tissue and muscle
  • silver impregnating reticulum
  • silver impregnating and staining neurological elements
  • periodic acid-Schiff (PAS) and Feulgen techniques and related reactions
  • staining haematologic elements and related tissues
  • staining pigments and minerals
  • staining proteins and nucleic acids
  • staining lipids and carbohydrates
  • staining cellular elements
  • staining golgi apparatus, mitochondria, and living cells
  • staining microorganisms. Part 5 Histochemistry and miscellaneous special procedures: histochemistry
  • immunohistochemical methods
  • microwave histology
  • special procedures I
  • special procedures II
  • special procedures III
  • special procedures. Part 6 Solution preparation and general laboratory aids: solution preparation
  • general laboratory aids. (Part contents)

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