Biochemical techniques in the heart

Bibliographic Information

Biochemical techniques in the heart

edited by John H. McNeill

(CRC Press methods in the life sciences, CRC Press methods in pharmacology)

CRC Press, c1997

  • alk. paper

Available at  / 2 libraries

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Note

Includes bibliographical references and index

Description and Table of Contents

Description

Biochemical Techniques in the Heart fully describes classical biochemical measurements of parameters involved in contraction and relaxation in the heart. This fully detailed guide tells you how to make preparations of sarcoplasmic reticulum, sarcolemma, and cardiomyocytes and how to measure sodium-calcium and sodium-hydrogen exchange. Two chapters explain the measurement of the important enzyme sodium-potassium ATPase. This book examines the most widely used tools in experimental cardiology and provides you with the recipe-setting up the technique, procurement of equipment, sample data and calculations, problems and trouble shooting, adapting to other species, modifications, and applicability. Undoubtedly, this text will be a great asset to cardiovascular physiologists, pharmacologists, experimental cardiologists, and students of physiology and pharmacology.

Table of Contents

Preparation of Cardiac Sarcoplasmic Reticulum, J.J. Leddy and B.S. Tauna The Isolation of Myocardial Sarcolemma, G.F. Tibbits Measurement of Na+-Ca2+ Exchange, G.F. Tibbits Measurement of Sodium/Potassium ATPase in Myocardium, V. Elimban, A. Lukas, and N.S. Dhalla Molecular Assessment of Cardiac Sodium Pump Activity, V. Elimban, K. Kato, and N.S. Dhalla Methods for Measuring Sodium-Hydrogen Exchange in the Heart, H. Massaeli and G.N. Pierce Preparation of Cardiomyocytes, B. Rodrigues and D.L. Severson

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