Interleukin protocols

著者

• O'Neill, Luke
• Bowie, Andrew

書誌事項

Interleukin protocols

edited by Luke A.J. O'Neill and Andrew Bowie

（Methods in molecular medicine / John M. Walker, series editor, 60）

Humana Press, c2001

• alk. paper

注記

Includes bibliographical references and index

内容説明・目次

内容説明

Interleukins are a family of proteins that regulate the maturation, diff- entiation, or activation of cells involved in immunity and inflammation, and belong to a broader family termed cytokines. Collectively these proteins are the key orchestrators of host defense and the response to tissue injury. There are currently 23 different interleukins (numbered from IL-1 to IL-23), although the full extent of the interleukin family will only become clear upon analysis of the human genome sequence. Most important, interleukins are central to the pathogenesis of a wide range of diseases that involve an immune com- nent, including such conditions as rheumatoid arthritis, multiple sclerosis, ulcerative colitis, psoriasis, and asthma. Interleukins have also been imp- cated in other conditions, including cancer, migraine, myocardial infarction, and depression. In essence, when cells are activated by interleukins, a program of gene expression is initiated in the target cell that alters the cell's phenotype, leading to enhanced immune reactivity, inflammation, and/or proliferation. Interleukins are therefore at the core of the cellular basis for many diseases. They are the subject of intense investigation by biomedical researchers and the targeting or use of interleukins in the clinic is proceeding apace. Approaches such as t- geting IL-4 in asthma or IL-1 in joint disease are being pursued, and it is likely that in the next 5-10 years a number of new therapies based on either inhib- ing or administering interleukins will be available.

目次

Part I: General Methods for Assaying Interleukin Protein and mRNA ELISAs and Interleukin Research Catherine Greene ELISPOT Technique for Assaying Interleukins Almuth Christine Hauer and Mona Bajaj-Elliott Measurement of Interleukins by ELISPOT Assay with Particular Application for Dual Color Analysis (Stardust Assay) Yoshihiro Okamato and Mikio Nishida Assaying Interleukins Intracellularly by FACS Liam O'Mahony and Maurice O'Donoghue Basic RT-PCR for Measurement of Cytokine Expression Mona Bajaj-Elliot and Almuth Christine Hauer Quantitative PCR for Measurement of Cytokine Expression Ellen M. Nilsen, Finn-Eirik Johnasen, and Per Brandtzaeg Single Cell RT-PCR Method for the Detection of Cytokine mRNA Moshe Ligumsky, Hovav Nechushtan, and Ehud Razin. uantitation of Cytokine mRNA by Flash-Type Bioluminescence Jeffrey K. Actor Measurement of Interleukin mRNA by Northern Blotting Using a Nonradioactive Detection Method Ginette Tardif, John A. Di Battista, Jean-Pierre Pelletier, and Johanne Martel-Pelletier Quantification of Mouse IL-6 and TNF-_ mRNA Using Xplore Assays Scott W. Van Arsdell, Kevin P. Murphy, Csaba Pazmany, Diane Erickson and Mark D. Moody Measurement of Cytokine and Chemokine mRNA Using Nonisotopic MultiProbe RNase Protection Assay Zheng-Ming Wang and Roman Dziarski Isolation of Total and Bioactive Interleukins by Immunoaffinity-Receptor Affinity Chromatography Terry M. Phillips and Benjamin F. Dickens Detection of Cytokines by Immunohistochemistry Mahmoud Huleihel Whole Blood Assays and the Influence of Circadian Rhythmicity on Human Cytokine Measurement Nicolai Petrovsky Part II: Illustration of Methodologies Using Individual Interleukins Assaying Interleukin-8 Paul N. Moynagh Measurement of Interleukin-2 by Bioassay Lyanne Weston Assaying Interleukin-15 Amos Douvdevani, Michal Weiler, and Eli Lewis Part III: Assaying Interleukins in Particular Pathologies Assaying Interleukin-6 in Breast Cancer Judith Harmey, Amanda Haverty, Deirdre Foley and David Bouchier-Hayes Cytokines, Stress, and Depressive Illness Hymie Anisman, Shawn Hayley, Arun V. Ravindran, Zul Merali, and Jenna Griffiths Measurement of Interleukins in Cutaneous Disorders Khusru Asadullah, Antje Haeussler-Quade, Wolf Dietrich Doecke, Wolfram Sterry Interleukins in Graves' Disease Jan Komorowski Evaluation of Cytokines in Migraine Patients by ELISA Irene Munno, Mariarosaria Marinaro, Giuseppe Lacedra, Antonia Bassi, and Vincenzo Centonze Assaying Interleukins in Plasma in the Course of Acute Myocardial Infarction Radek Pudil Part IV: Assaying Interleukins in Particular Biological Fluids Measurement of Cytokines in Peritoneal Fluids Reijo Punnonen and Juha Punnonen Measurement of Cytokines in Seminal Plasma Marek Glezerman, Eitan Lunenfeld, and Mahmoud Huleihel Measurement of Cytokines in Induced Sputum: Application to the Investigation of Asthma and COPD Peter G. Gibson and Jodie L. Simpson Cytokines in Blister Fluid Franco Ameglio and Luciano D'Auria Measurement of Cytokines in Synovial Fluid Fabrizio De Benedetti Measurement of Cytokines in Cerebrospinal Fluid Toshiyuki Yokoyama Detection of Interleukins in Tears Archana Thakur and Mark D. P. Willcox Part V: Newer Techniques: Interleukin Signal Transduction, Recombinant Interleukin Production, Interleukin Gene Polymorphisms, and the use of cDNA Arrays Measurement of Phosphoinositide 3-Kinase Activation by Interleukins Stephen G. Ward Analysis of Interleukin-2 S

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詳細情報

• NII書誌ID(NCID): BA60656423
• ISBN:
  • 089603738X
• LCCN: 2001016960
• 出版国コード: us
• タイトル言語コード: eng
• 本文言語コード: eng
• 出版地: Totowa, N.J.
• ページ数/冊数: xv, 443 p.
• 大きさ: 24 cm.
• 分類:
  • LCC : QR185.8.I56
  • DC21 : 616.07/9
• 件名:
  • LCSH : Interleukins -- Laboratory manuals
• 親書誌ID:
  • BA2811990X