Genetic recombination : reviews and protocols

著者

    • Waldman, Alan S.

書誌事項

Genetic recombination : reviews and protocols

edited by Alan S. Waldman

（Methods in molecular biology / John M. Walker, series editor, v. 262）

Humana Press, c2004

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注記

Includes bibliographical references and index

内容説明・目次

内容説明

Genetic recombination, in the broadest sense, can be defined as any process in which DNA sequences interact and undergo a transfer of information, producing new "recombinant" sequences that contain information from each of the original molecules. All organisms have the ability to carry out recombination, and this striking universality speaks to the essential role recombination plays in a variety of biological processes fundamentally important to the maintenance of life. Such processes include DNA repair, regulation of gene expression, disease etiology, meiotic chromosome segregation, and evolution. One important aspect of recombination is that it typically occurs only between sequences that display a high degree of sequence identity. The stringent requirement for homology helps to ensure that, under normal circumstances, a cell is protected from deleterious rearrangements since a swap of genetic information between two nearly identical sequences is not expected to dramatically alter a genome. Recombination between dissimilar sequences, which does happen on occasion, may have such harmful consequences as chromosomal translocations, deletions, or inversions. For many organisms, it is also important that recombination rates are not too high lest the genome become destabilized. Curiously, certain organisms, such as the trypanosome parasite, actually use a high rate of recombination at a particular locus in order to switch antigen expression continually and evade the host immune system effectively.

目次

Part I. Studying Recombination Events in Eukaryotes Determination of Mitotic Recombination Rates by Fluctuation Analysis in Saccharomyces cerevisiae Rachelle Miller Spell and Sue Jinks-Robertson Determination of Intrachromosomal Recombination Rates in Cultured Mammalian Cells Jason A. Smith and Alan S. Waldman Intrachromosomal Homologous Recombination in Arabidopsis thaliana Waltraud Schmidt-Puchta, Nadiya Orel, Anzhela Kyryk, and Holger Puchta Analysis of Recombinational Repair of DNA Double-Strand Breaks in Mammalian Cells With I-SceI Nuclease Jac A. Nickoloff and Mark A. Brenneman Transformation of Monomorphic and Pleomorphic Trypanosoma brucei Richard McCulloch, Erik Vassella, Peter Burton, Michael Boshart, and J. David Barry Forward Genetic Screens for Meiotic and Mitotic Recombination-Defective Mutants in Mice Laura Reinholdt, Terry Ashley, John Schimenti, and Naoko Shima Part II. Recombination as a Reporter of Genomic Instability Detecting Carcinogens With the Yeast DEL Assay Richard J. Brennan and Robert H. Schiestl In Vivo DNA Deletion Assay to Detect Environmental and Genetic Predisposition to Cancer Ramune Reliene, Alexander J. R. Bishop, Jiri Aubrecht, and Robert H. Schiestl Part III. Recombination as a Tool for Producing Targeted Genetic Modification Gene Targeting at the Chromosomal Immunoglobulin Locus: A Model System for the Study of Mammalian Homologous Recombination Mechanisms Mark D. Baker DNA Fragment Transplacement in Saccharomyces cerevisiae: Some Genetic Considerations Glenn M. Manthey, Michelle S. Navarro, and Adam M. Bailis Targeted Gene Modification Using Triplex-Forming Oligonucleotides Jean Y. Kuan and Peter M. Glazer Using Nucleases to Stimulate Homologous Recombination Dana Carroll Enhancement of In Vivo Targeted Nucleotide Exchange by Nonspecific Carrier DNA Katie K. Maguire and Eric B. Kmiec Part IV. Biochemistry of Recombination Chromatin Immunoprecipitation to Investigate Protein-DNA Interactions During Genetic Recombination Tamara Goldfarb and Eric Alani Holliday Junction Branch Migration and Resolution Assays Angelos Constantinou and Stephen C. West Index

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