Environmental microbiology : a laboratory manual

Author(s)

• Pepper, Ian L.
• Gerba, Charles P.

Bibliographic Information

Environmental microbiology : a laboratory manual

I.L. Pepper and C.P. Gerba

Elsevier academic Press, 2004

2nd ed

Note

Includes bibliographical references

Description and Table of Contents

Description

Environmental Microbiology: A Laboratory Manual is designed to meet the diverse requirements of upper division and graduate-level laboratory sessions in environmental microbiology. The experiments introduce students to the activities of various organisms and the analyses used to study them. The book is organized into three thematic sections: Soil Microbiology, Water Microbiology, and Environmental Biotechnology. The first section includes experiments on the soil as a habitat for microorganisms, and introduces the main types of soil microorganisms, how they interact with the soil, and the techniques used in their analysis. Experiments in the second section cover assays of microbial pathogens--bacteria, viruses, and protozoan parasites--used in food and water quality control as well as an exercise in applied bioremediation of contaminants in water. The final section on biotechnology includes applications of the polymerase chain reaction (PCR) for the detection of bacteria and the use of enrichment cultures and a computer-based, physiological test bank to isolate and identify a bacterium useful in bioremediation. Designed for maximum versatility and ease of use for both the student and instructor, each experiment is self-contained and includes theoretical, practical, and pedagogical material.

Table of Contents

Preface Basic Protocols Experiment 1 - Dilution and Plating of Bacteria and Growth Curves Experiment 2 - Soil Moisture Content Determination Examination of Soil Microorganisms via Microscopic and Cultural Assays Experiment 3 - Contact Slide Assay Experiment 4 - Filamentous Fungi Experiment 5 - Bacteria and Actinomycetes Experiment 6 - Algae: Enumeration by MPN Microbial Transformations and Response to Contaminants Experiment 7 - Oxidation of Sulfur in Soil Experiment 8 - Dehydrogenase Activity of Soils Experiment 9 - Nitrification and Denitrification Experiment 10 - Enrichment and Isolation of Bacteria that Degrade 2, 4-dichlorophenoxyacetic acid Experiment 11 - Adaptation of Soil Bacteria to Metals Experiment 12 - Biodegradation of Phenol Compounds Experiment 13 - Assimilable Organic Matter Experiment 14 - Biochemical Oxygen Demand Water Microbiology Experiment 15 - Membrane Filter Technique Experiment 16 - Bacteriological Examination of Water: the Coliform MPN Test Experiment 17 - Detection of Bacteriophages Experiment 18 - Film Medium for the Detection of Coliforms in Water, Food, and on Surfaces Experiment 19 - Defined Substrate Technology for the Detection of Coliforms and Fecal Coliforms Advanced Topics Experiment 20 - Detection of Enteric Viruses in Water Experiment 21 - Detection of Waterborne Parasites Experiment 22 - Kinetics of Disinfection Experiment 23 - Aerobiology: Sampling of Airborne Microorganisms Experiment 24 - Detection and Identification of Bacteria via PCR and Subsequent BLAST Analysis of Amplified Sequences Appendix 1: Preparation of Media and Stains for Each Experiment Appendix 2: Glossary

by "Nielsen BookData"

Details

• NCID: BA71099169
• ISBN:
  • 0125506562
• Country Code: us
• Title Language Code: eng
• Text Language Code: eng
• Place of Publication: Amsterdam
• Pages/Volumes: xvii, 209 p.
• Size: 28 cm
• Classification:
  • NDC8 : 465
  • LCC : QR100
• Subject Headings:
  • LCSH : Microbial ecology -- Laboratory manuals