Biological mass spectrometry
著者
書誌事項
Biological mass spectrometry
(Methods in enzymology / editors in chief, Sidney P. Colowick, Nathan O. Kaplan, v. 402)
Elsevier Academic Press, c2005
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注記
Includes bibliographical references and indexes
内容説明・目次
内容説明
This volume describes and integrates the techniques and fundamentals of more than a decade of revolutionary advances in both chromatographic and mass spectrometric technologies that have enabled the direct investigation of biomacromolecules per se and have provided the analytical power base to usher in the new fields of proteomics and systems biology. It also covers new biophysical applications such as H/D exchange for study of conformations, protein-protein and protein-metal and ligand interactions. Finally it describes atto-to-zepto-mole quantitation of 14C and 3H by accelerator mass spectrometry.
目次
- Mass Spectrometers for the Analysis of Biomolecules
- Hybrid Quadrupole / Time-of-Flight Mass Spectrometers for Analysis of Biomolecules
- Tandem Time-of-Flight Mass Spectrometry
- Tandem Mass Spectrometry in Quadrupole Ion Trap and Ion Cyclotron Resonance
- Collision-Induced Dissociation (CID) of Peptides and Proteins
- Peptide Sequencing by MALDI 193-nm Photodissociation TOF MS
- Peptide Sequence Analysis
- Proteomics
- Bioinformatic methods to exploit mass spectrometric data for proteomic applications
- Protein Conformations, Interactions and H/D Exchange
- Ligand/ Metal Ion Binding to Proteins: Investigation by ESI Mass Spectrometry
- Site specific hydrogen exchange of proteins: Insights into the structures of Amyloidogenic intermediates
- Quantitating Isotopic Molecular Labels with Accelerator Mass Spectrometry
- Accelerator Mass Spectrometry for Biomedical Research
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