Lipidomics and bioactive lipids : lipids and cell signaling

著者

• Brown, H. Alex

書誌事項

Lipidomics and bioactive lipids : lipids and cell signaling

edited by H. Alex Brown

（Methods in enzymology / editors in chief, Sidney P. Colowick, Nathan O. Kaplan, v. 434）

Elsevier Academic Press, c2007

注記

Includes bibliographical references and indexes

内容説明・目次

内容説明

This volume in the well-established Methods in Enzymology series features methods for the study of lipids using mass spectrometry techniques. Articles in this volume cover topics such as Phospholipase A1 assays using a radio-labeled substrate and mass spectrometry; Real-time Cell Assays of Phospholipases A2 Using Fluorogenic Phospholipids; Analysis and Pharmacological Targeting of Phospholipase C a interactions with G proteins; Biochemical Analysis of Phospholipase D.; Measurement of Autotaxin/Lysophospholipase D Activity; Platelet-Activating Factor; Quantitative measurement of PtdIns(3,4,5)P3; Measuring Phosphorylated Akt And Other Phosphoinositide 3-Kinase-Regulated Phosphoproteins In Primary Lymphocytes; Regulation of Phosphatidylinositol 4-Phosphate 5-Kinase activity by partner proteins; Biochemical Analysis of Inositol Phosphate Kinases; Analysis of the phosphoinositides and their aqueous metabolites; Combination of C17-sphingoid base homologues and mass spectrometry analysis as a new approach to study sphingolipid metabolism; Measurement of mammalian sphingosine-1-phosphate phosphohydrolase activity in vitro and in vivo; A rapid and sensitive method to measure secretion of sphingosine-1-phosphate; Ceramide Kinase and Ceramide-1-Phosphate; Measurement of Mammalian Diacylglycerol Kinase Activity in vitro and in Cells; Lipid Phosphate Phosphatases from Saccharomyces cerevisiae.

目次

Chapter 1 Phospholipase A1 assays using a radio-labeled substrate and mass spectrometry REI MORIKAWA, MASAFUMI TSUJIMOTO, HIROYUKI ARAI and JUNKEN AOKI Chapter 2 Real-time Cell Assays of Phospholipases A2 Using Fluorogenic Phospholipids Debasis Manna, Wonhwa Cho Chapter 3 Analysis and Pharmacological Targeting of Phospholipase C a interactions with G proteins David M. Lehmann, Chujun Yuan and Alan V. Smrcka Chapter 4 Biochemical Analysis of Phospholipase D. H. Alex Brown, Lee G. Henage, Anita M. Preininger, Yun Xiang, John H. Exton Chapter 5 Measurement of Autotaxin/Lysophospholipase D Activity Andrew J. Morris and Susan S. Smyth Chapter 6 Platelet-Activating Factor John S. Owen, Michael J. Thomas, and Robert L. Wykle Chapter 7 Quantitative measurement of PtdIns(3,4,5)P3 H. Guillou, L. Stephens, P.T. Hawkins Chapter 8 Measuring Phosphorylated Akt And Other Phosphoinositide 3-Kinase-Regulated Phosphoproteins In Primary Lymphocytes Amber C. Donahue, Michael G. Kharas, and David A. Fruman Chapter 9 Regulation of Phosphatidylinositol 4-Phosphate 5-Kinase activity by partner proteins Yasunori Kanaho, Kazuhisa Nakayama, Michael A. Frohman, and Takeaki Yokozeki Chapter 10 Biochemical Analysis of Inositol Phosphate Kinases James C. Otto, Sashidhar Mulugu, Peter C. Fridy, Shean-Tai Chiou, Blaine N. Armbruster, Anthony A. Ribeiro and John D. York Chapter 11 Analysis of the phosphoinositides and their aqueous metabolites Christopher P. Berrie, Cristiano Iurisci, Enza Piccolo, Renzo Bagnati and Daniela Corda Chapter 12 Combination of C17-sphingoid base homologues and mass spectrometry analysis as a new approach to study sphingolipid metabolism. Stefka Spassieva, Jacek Bielawski, Viviana Anelli, and Lina M. Obeid Chapter 13 Measurement of mammalian sphingosine-1-phosphate phosphohydrolase activity in vitro and in vivo Michael Maceyka, Sheldon Milstien, Sarah Spiegel Chapter 14 A rapid and sensitive method to measure secretion of sphingosine-1-phosphate Poulami Mitra, Shawn G. Payne, Sheldon Milstien, Sarah Spiegel Chapter 15 Ceramide Kinase and Ceramide-1-Phosphate Dayanjan S. Wijesinghe, Nadia F. Lamour, Antonio Gomez-Munoz, and Charles E. Chalfant Chapter 16 Measurement of Mammalian Diacylglycerol Kinase Activity in vitro and in Cells Richard M. Epand and Matthew K. Topham Chapter 17 Lipid Phosphate Phosphatases from Saccharomyces cerevisiae George M. Carman and Wen-I Wu

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詳細情報

• NII書誌ID(NCID): BA83769509
• ISBN:
  • 9780123739650
• 出版国コード: ne
• タイトル言語コード: eng
• 本文言語コード: eng
• 出版地: Amsterdam ; Tokyo
• ページ数/冊数: xliv, 342 p., [8] p. of plates
• 大きさ: 24 cm
• 親書誌ID:
  • BA00197543